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Noriaki Nagai, Yu Mano, Hiroko Otake, Teppei Shibata, Eri Kubo, Hiroshi Sasaki; Amyloid β1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients. Invest. Ophthalmol. Vis. Sci. 2017;58(7):3294-3302. doi: https://doi.org/10.1167/iovs.17-22065.
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© ARVO (1962-2015); The Authors (2016-present)
We investigated the accumulation of amyloid β (Aβ1–40, Aβ1–42, Aβ1–43) in the lens epithelium of patients with opacification of five different types (cortical cataract [COR]; nuclear cataract [NUC]; posterior subcapsular cataract [PSC]; retrodots [RD]; and water clefts [WC]).
Samples were collected from Japanese patients taken during cataract surgery; Aβ levels and mRNA expression were determined by ELISA and a real-time RT-PCR method, respectively.
Levels of Aβ1–40 and Aβ1–42 in the lens epithelium of patients with COR, NUC, PSC, RD, and WC showed no significant differences in comparison with transparent lens epithelium. Levels of Aβ1–43 in the lens epithelium of patients with PSC and WC were not detected, and NUC and RD were slightly elevated. In contrast to the results in these cataract types, high Aβ1–43 levels were observed in the lens epithelium of patients with COR, and a close relationship was observed between Aβ1–43 levels and the degree of lens opacification (R = 0.8229, n = 6). The levels of Aβ1–43 were also higher in the lens epithelium of patients with mixed-cataract showing cortical opacification, and the Aβ1–43 levels in the lens epithelium of mixed-cataract patients with cortical opacification was significantly higher than in that of mixed-cataract patients without cortical opacification. In addition, the level of an amyloid precursor protein mRNA in the lens epithelium of mixed-cataract patients with cortical opacification was significantly higher than in transparent lens and mixed-cataract patients without cortical opacification.
We found high levels of Aβ1–43 accumulation in the lens epithelium of Japanese patients with cortical opacification.
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