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Matilda F Chan, Catherine Oldenburg, Jacquelyn Kemmer, Selene M. Clay, David G. Hwang, Marie Wolf; Overexpression of MMPs in Corneas Requiring Penetrating and Deep Anterior Lamellar Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2017;58(8):138.
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© ARVO (1962-2015); The Authors (2016-present)
Matrix metalloproteinases (MMPs) comprise a family of zinc-dependent endopeptidases which are involved in wound healing processes including neovascularization and fibrosis. We wanted to assess MMP protein expression levels in diseased corneas of patients requiring penetrating and deep anterior lamellar keratoplasty. The purpose of this study is to test the hypothesis that up-regulation of MMPs in diseased corneas is correlated with clinical levels of corneal neovascularization and fibrosis.
Protein expression levels of 9 individual MMPs were detected and quantified simultaneously in human corneal lysates using the Bio-Plex Pro Human MMP 9-Plex Panel and the MAGPIX technology. Differences in expression patterns of MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP12 and MMP13 were assessed between diseased specimens obtained from 21 patients undergoing corneal transplantation (17 penetrating keratoplasty and 4 deep anterior lamellar keratoplasty) and 6 eye bank control corneas. MMP expression levels were correlated with clinical data of the patients.
Of the 21 diseased patient samples, 14 (67%) are male and the mean age is 56 years. For the control samples, 4 (67%) are male and the mean age is 61 years. Luminex based expression analysis revealed a significant overexpression of 5 of the 9 MMPs tested (MMPs 1, 2, 8, 12, 13) compared to the control group. Significant overexpression of MMP1, MMP12, and MMP13 was observed in diseased corneas with neovascularization compared with diseased corneas without neovascularization. Overexpression of MMPs 1, 2, 8, 12, and 13 also corresponded with the levels of corneal fibrosis.
The overexpression of several MMPs (MMPs 1, 2, 8, 12, 13) occurs in corneas from patients requiring keratoplasty. The expression of these MMPs positively correlates with clinical levels of corneal neovascularization and fibrosis and supports an important role of MMPs in these repair processes.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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