Purchase this article with an account.
Dorette Z Ellis, Linya Li, Shaoqing He, Yang Liu, Thomas Yorio; Activation of Sigma-1 Receptor Protects Retinal Ganglion Cell Loss in Optic Nerve Crush Model for Glaucoma. Invest. Ophthalmol. Vis. Sci. 2017;58(8):212.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Recent studies in sigma-1 receptors (σ-1r) deficient (k/o) mice demonstrated increased losses of retinal ganglion cells (RGCs) in oxidative stress and optic nerve crush mice models (ONC). This study determines the neuroprotective role of the σ-1r in ONC model.
ONC was performed in σ-1r k/o mice and wild type mice. Briefly, the left optic nerve (ON) was exposed intraorbitally through a small window made between the surrounding muscles. The ON was crushed approximately 1mm behind the globe with self-closing forceps for 4 seconds under visualization. The contralateral eye was used as controls for ONC and σ-1r k/o mice served as controls for off receptor action. The σ-1r was activated by the intraperitoneal injection with pentazocine (PTZ; 0.5 mg/kg) 3x weekly for the duration of the experiments. Additionally, two weeks prior to ONC, mice were intravitreally injected with AAV2-CAg-σ-1r-GFP vector and expression of σ-1r was assessed; AAV2 empty vector served as controls. RGC activity was measured using pattern electroretinogram (pERG). Termination experiments included counting RGCs in experimental animals to determine neuroprotective activities of the σ-1r.
RGC death was decreased in wild-type mice treated with PTZ when compared with σ-1r k/o or untreated animals. σ-1r k/o ONC mice injected with AAV2-CAg-σ-1r-GFP vector demonstrated significant increases in RGCs numbers and activity when compared with σ-1r k/o ONC mice injected with empty vector or non-injected σ-1r k/o ONC animals.
Our studies indicate that expression or activation of σ-1rs provides neuroprotection of RGCs.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
This PDF is available to Subscribers Only