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Vijaya Juturu, Fatih Akdemir, Cemal Orhan, Mehmet Tuzcu, Nurhan Sahin, Ismet Yilmaz, Kazim Sahin; Lutein and Zeaxanthin isomers (L/Zi) Modulate Photo-Oxidative Retinal Damage in an Animal Model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):240.
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© ARVO (1962-2015); The Authors (2016-present)
Lutein and Zeaxanthin isomers (L/Zi) able to protect against photoreceptor cell degeneration and neutralize free radicals produced by oxidative stress. We tested this hypothesis to determine the effects of L/Zi in ocular tissues and plasma of rats exposed to dark and intense light.
Male Wistar rats (age: 8 weeks, weight: 180 ± 20 g) were housed in a controlled environment with a 12:12-h light-dark cycle at 22°C and provided with rat chow and water ad libitum. All experiments were conducted under the National Institutes of Health's Guidelines for the Care and Use of Laboratory Animals and approved by the Ethics Committee of the Inonu University. Rats were randomly divided into treatment groups (with and without L/Zi supplementation) and exposed to intense light (LED, light [L] and dark [D] adaptation). Group I rats exposed to 12 h L and 12 h D (+/- 100 mg L/Zi/kg BW); Group II to 16 h L and 8 h (+/- 100 mg L/Zi/kg BW) and Group II exposed to 24 h L (+/- 100 mg L/Zi/kg BW) for 60 days. Vision and brain health markers such as Rhodopsin (Rho) Rod arrestin (Sag), G Protein Subunit Alpha Transducin 1 (Gnat1), nuclear factor-kappa B (NFkB), neural cell adhesion molecule (NCAM), growth-associated protein-43 (GAP43) , glial fibrillary acid protein (GFAP), nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and Heme oxygenase 1 (HO-1) were analyzed. Oxidative stress and anti -inflammatory markers were also analyzed. The data were analyzed using the GLM procedure of SAS (SAS Institute: SAS User’s Guide). The treatments were compared using ANOVA and student's unpaired t test; P < 0.05 was considered statistically significant.
Increased NFkB and GFAP and decreased Rho, Rod arrestin (Sag), Gnat1 , NCAM, GAP43 , Nrf2 and HO-1 observed in 24 h light intensity adaptation followed by 16h IL and 8 h DA. L/Zi administration significantly alleviated oxidative stress (NFkB and GFAP) and improved antioxidant capacity gene proteins (Rho, Rod arrestin (Sag), Gnat1, NCAM, GAP43, Nrf2 and HO-1) in retinal tissues. SOD and GSH-Px improved with L/Zi. No gross lesions, no mortality and no adverse events were observed.
These observations suggest that L/Zi may be considered as an adjunct therapy to prevent photoreceptor cell degeneration and neutralize free radicals produced by oxidative stress and visual health.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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