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MARCO Otavio Santos de ALMEIDA, Fábio Ursulino Reis Carvalho, Obidulho Sakassegawa Naves, Andre Ferreira da Silva, Ricardo Holzchuh, Richard Y Hida, Fernando Cesar Abib; Analysis of the age compatibility of donor corneal endothelium: a pilot study.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1452.
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© ARVO (1962-2015); The Authors (2016-present)
Eye Banks utilize specular microscopy (SM) technology to proper evaluate the corneal endothelium. Most of them use a fixed cell density cut-off value to consider a cornea suitable for optical transplantation, regardless of the donor’s age. This study aims to analyze the variation of the parameters obtained from each donor cornea's endothelial images in relation to their age compatibility.
Seven donor corneal tissues were examined in their arrival day (group 1) and their distribution day (group 2) in our eye bank using specular microscopy (Kerato Analyzer EKA-10, KONAN©). All corneas were preserved in Eusol-C at a temperature of 4 degress celsius and heated to 27 degress celsius before examination. Four endothelial images (EI) of each donor cornea were analyzed in the semi-automated method and the manufacturer’s software (KONAN KSS-EB10) produced a multi-sample average (image 1). Cells Analyzer® software (Corneal Endothelial Lab, Technicall, Brazil) was used to obtain age compatibility of each parameter: cell density (CD), average cell area (AVE), coefficient of variation (CV) and hexagonal cells percentage (HEX). Descriptive statistics was used for statistical analysis.
Mean donors age was 57,14 (±6,12), ranging from 52 to 69 years. Corneal storage time was 1,86 days (±1,21). The data obtained is shown in tables 1 and 2 (displayed side by side for better comparison). In group 1, 75% of the EI presented CD outside of the values considered normal for age. In addition, 75%, 85.71% and 82.14% of the EI showed AVE, CV and HEX incompatible with their age, respectively. In group 2, 85.71%, 82.12%, 100% and 96.42% of the EI showed CD, AVE, CV and HEX outside of the normal values for age, respectively. The same eye presented images with CD inside and outside normal values for age in 42.85% of the corneas in group 1 and 28.57% in group 2.
Age incompatibility was observed in all specular microscopy parameters. Although a fixed threshold value is useful, the analysis of the CD age compatibility of donor corneas could help in better selecting the most suitable receptors.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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