June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Validation of an adaptive optics scanning laser ophthalmoscope prototype in patients with degenerative retinal diseases.The LITE study.
Author Affiliations & Notes
  • LUCIA LEE FERRARO
    Institut de la Macula, Barcelona, Spain
    Barcelona Macula Foundation, Barcelona, Spain
  • david merino
    ICFO Institute of Photonic Sciences, Barcelona, Spain
  • Marc Biarnes Perez
    Institut de la Macula, Barcelona, Spain
    Barcelona Macula Foundation, Barcelona, Spain
  • Miriam Garcia Planas
    Institut de la Macula, Barcelona, Spain
    Barcelona Macula Foundation, Barcelona, Spain
  • Anna Borrell
    Barcelona Macula Foundation, Barcelona, Spain
  • Pablo Loza
    ICFO Institute of Photonic Sciences, Barcelona, Spain
  • Jordi Monés
    Institut de la Macula, Barcelona, Spain
    Barcelona Macula Foundation, Barcelona, Spain
  • Footnotes
    Commercial Relationships   LUCIA LEE FERRARO, The 7th Framework Programme of the European Union (F); david merino, None; Marc Biarnes Perez, The 7th Framework Programme of the European Union (F); Miriam Garcia Planas, The 7th Framework Programme of the European Union (F); Anna Borrell, The 7th Framework Programme of the European Union (F); Pablo Loza, None; Jordi Monés, The 7th Framework Programme of the European Union (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 309. doi:
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      LUCIA LEE FERRARO, david merino, Marc Biarnes Perez, Miriam Garcia Planas, Anna Borrell, Pablo Loza, Jordi Monés; Validation of an adaptive optics scanning laser ophthalmoscope prototype in patients with degenerative retinal diseases.The LITE study.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):309.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To report the clinical validation of a newly designed and implemented confocal adaptive optics scanning laser ophthalmoscope (AOSLO) device in the context of the LITE (“Development of advanced laser imaging techniques for the anterior and posterior eye”) study.

Methods : A prospective,observational,1-year study was conducted at the Institut de la Màcula (Barcelona, Spain) between August 2015 and November 2016 to validate the instrument. After testing the device in healthy subjects, patients with Stargardt’s disease(STG) or retinitis pigmentosa(RP) were enrolled and visited at baseline, 3, 6 and 12 months. At each visit complete ophthalmic exam and retinal imaging were performed, in addition to AOSLO in regions of interest.

Results : Twenty-two patients were screened and 16 met the eligibility criteria: 5 healthy, 6 with STG and 5 with RP. Patients with degenerative diseases had a median of 47 years old (range, 35 to 64), there were 45.5% (5/11) females and 81.8% (9/11) were right eyes. Median baseline best-corrected visual acuity (BCVA) was 78 letters (range, 54 to 91) and median macular sensitivity was 16.8 dB (range, 3.5 to 27.8). AOSLO imaged a regular mosaic of photoreceptors in healthy individuals (Figure 1). In STG and RP patients (Figure 2) AOSLO showed irregular mosaics even in normal-appearing areas, according to fundus autofluorescence(FAF) and/or spectral domain optical coherence tomography(SD-OCT). These findings highlight the sensitivity of AOSLO imaging tools for assessing retina mosaic structure; moreover, damage to the outer retinal layers identified on AOSLO precedes that seen with current state-of-the-art instruments.

Conclusions : The newly developed AOSLO provided high-quality images of the photoreceptors(or lack thereof) in STG and RP, and may provide insights into disease progression and pathogenesis.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

AOSLO image of a healthy patient at 0,5° in the superior meridian.

AOSLO image of a healthy patient at 0,5° in the superior meridian.

 

Images of a patient with RP. FAF(A)-guided SD-OCT(B) images at Month6, showing near-normal outer retinal appearance inside the hyperreflective ring(blue arrowhead). AOSLO images of the blue square at Month0 (C) and Month6 (D) show irregular retinal mosaic. FAF(E)-guided SD-OCT(F) images at Month6, showing loss of outer retinal layers in the hyperreflective ring (red arrowhead). AOSLO images of the red square at Month0 (G) and Month6 (H) show progressive loss of retinal details.

Images of a patient with RP. FAF(A)-guided SD-OCT(B) images at Month6, showing near-normal outer retinal appearance inside the hyperreflective ring(blue arrowhead). AOSLO images of the blue square at Month0 (C) and Month6 (D) show irregular retinal mosaic. FAF(E)-guided SD-OCT(F) images at Month6, showing loss of outer retinal layers in the hyperreflective ring (red arrowhead). AOSLO images of the red square at Month0 (G) and Month6 (H) show progressive loss of retinal details.

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