June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Retinal Ganglion Cell layer by Fourier-domain Optical Coherence Tomography and microvasculature density by Optical Coherence Tomography Angiography at the macular region in glaucoma.
Author Affiliations & Notes
  • Luis Silva
    Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, New York, New York, United States
  • Yanin Suwan
    Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, New York, New York, United States
    Ophthalmology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
  • Ravivarn Jarukasetphon
    Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, New York, New York, United States
  • Rashmi Rajshekhar
    Psychology, Columbia University, New York, New York, United States
  • C Gustavo De Moraes
    Ophthalmology, Columbia University, New York, New York, United States
  • Donald Hood
    Psychology, Columbia University, New York, New York, United States
    Ophthalmology, Columbia University, New York, New York, United States
  • Robert Ritch
    Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, New York, New York, United States
  • Footnotes
    Commercial Relationships   Luis Silva, None; Yanin Suwan, None; Ravivarn Jarukasetphon, None; Rashmi Rajshekhar, None; C Gustavo De Moraes, None; Donald Hood, Heidelberg Engineering (F), Topcon Corp (F); Robert Ritch, None
  • Footnotes
    Support  Supported in part by the Lary Stromfeld Glaucoma Research Fund of the New York Eye and Ear Infirmary of Mount Sinai.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 712. doi:
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      Luis Silva, Yanin Suwan, Ravivarn Jarukasetphon, Rashmi Rajshekhar, C Gustavo De Moraes, Donald Hood, Robert Ritch; Retinal Ganglion Cell layer by Fourier-domain Optical Coherence Tomography and microvasculature density by Optical Coherence Tomography Angiography at the macular region in glaucoma.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):712.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare the vascular perfusion in the macular area with the retinal ganglion cell (RGC) density in patients with glaucoma and suspects.

Methods : We performed a retrospective, observational clinical study to compare the RGC density obtained with fourier-domain optical coherence tomography (OCT - 3D-OCT, Topcon Corp), with macular blood vessel density determined by OCT angiography (OCTA - AngioVue, Optovue), in 32 eyes with open-angle glaucoma, 2 with exfoliation syndrome and ocular hypertension, and 8 glaucoma suspect eyes, based on the appearance of the optic disc (mean age: 65.66 ± 12.2 years, MD: -4.64 ± 5.05 dB). The superficial vascular layer located between the inner limiting membrane (ILM) and the posterior boundary of the RNFL was analyzed with OCTA AngioAnalytics software. OCT and OCTA (3 x 3 mm scans) images were aligned, and OCT RGC thickness and OCTA blood vessel density were compared by 7 regions – superior, nasal, inferior and temporal quadrants, superior and inferior hemifields, as well as the fovea (within 1mm from the foveola). All OCTA images with inadequate segmentation of the retinal layers, a signal strength index of <40, motion artifacts or significant macular epiretinal membranes were excluded from analysis.

Results : Mixed effects modelling revealed a significant relationship between the RGC+ and vascular density in the fovea (P < 0.005), inferior quadrant (P < 0.001) and average inferior hemifield (P < 0.005). OCTA color-coded density maps and gray scale images showed correlation with the color-coded RGC+ OCT maps (see figure 1). No significant relationship was seen in the nasal and temporal quadrants.

Conclusions : Glaucoma affects the RGC density in the macula, and the decreased blood supply at the affected area could be the result or consequence of glaucomatous damage. Previous studies have shown a correlation between a decreased RNFL thickness and a loss of blood vessels in the peripapillary region (Scripsema et al. IOVS 2016, Yarmohammadi et al. IOVS 2016). We demonstrated that this correlation is also present at the macula.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

Figure 1. Color Coded RGC+ (A), OCTA Images Gray Scale (B) and Color Coded Perfused Capillary Density Map (C)
1A-B-C: Normal, 2A-B-C: NTG, mild damage, 3A-B-C: NTG, moderate damage.
NTG = Normal Tension Glaucoma.

Figure 1. Color Coded RGC+ (A), OCTA Images Gray Scale (B) and Color Coded Perfused Capillary Density Map (C)
1A-B-C: Normal, 2A-B-C: NTG, mild damage, 3A-B-C: NTG, moderate damage.
NTG = Normal Tension Glaucoma.

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