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Shin-cihi Ikeda, Toshihide Kurihara, Xiaoyan Jiang, YASUHISA TANAKA, Kiwako Mori, Maki Miyauchi, Hidemasa Torii, Kazuo Tsubota; Morphological changes of scleral fibroblasts in a murine lens-induced myopia model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1115. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
A scleral remodeling is required for axial length elongation in the myopia development; however, the cellular components and the molecular mechanisms in the scleral remodeling have not well known. In this study, we tried to uncover the biological changes in the sclera especially focusing on the morphology in scleral fibroblasts during myopia development.
Male C57BL/6J mice (3 weeks old, n=5) were subjected to wear -30 diopter (D) lens on right eye and 0 D lens on left eye, respectively. After 3 weeks wearing, the refraction and the axial length were measured using a refractometer and a SD-OCT system in both eyes. After the measurements, eye balls were enucleated and fixed by 2.5 % glutaraldehyde in PBS to be prepared for ultra-thin sections, and observed with transmission electron microscopy (TEM).
TEM observation revealed that mouse sclera was mostly composed of collagen fibers and fibroblasts. Minus lens-worn eyes showed a refractive error shift (means ± standard deviation, 0D eyes: 1.10±1.24 D, -30D eyes: -13.44±5.75 D) and an axial length elongation (delta increase of axial length, 0D eyes: 0.09±0.02 mm, -30 D eyes: 0.26±0.07 mm) indicating a successful induction of myopia in C57BL6J mice. In 0 D lens-worn eyes, fibroblasts have rich mitochondria and rough endoplasmic reticulum (rough-ER). On the other hand, in -30 D lens-worn eyes, expanded ERs were observed in a large number of fibroblasts suggesting that ER stress may be induced in myopic sclera. Furthermore, intercellular contacts (macrophage-fibroblast, fibroblast-fibroblast) were observed in lens-induced myopic sclera especially in the inner lesion.
In myopic sclera, scleral fibroblasts showed 1) an enhanced ER stress appearance and 2) an increase in intercellular contacts. These phenotypical changes might be associated with the mechanism of the myopia development.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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