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Toshiaki Hirakata, Kentetsu Lee, Mai Ohba, Akira Matsuda, Akira Murakami, Takehiko Yokomizo; The effect of dietary omega-3 fatty acids on allergic conjunctivitis in mice.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):844. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Allergic conjunctivitis is one of the most common diseases of ocular surface. Previous studies showed that omega-3 fatty acids have anti-allergic and anti-inflammatory properties. It is unknown, however, whether omega-3 fatty acids have any therapeutic effect on allergic conjunctivitis. Thus, we evaluated the effectiveness of feeding of omega-3 fatty acids using a mouse model of allergic conjunctivitis.
BALB/c mice were fed either an omega-6 rich control diet or an omega-3 rich diet for one month. The mice were sensitized twice with Ragweed pollen (RW) in alum adjuvant and challenged with RW in eye drops. Clinical score was evaluated and eosinophil infiltration into the conjunctiva was counted. Levels of serum immunoglobulin E and cytokines in the conjunctival tissues were quantified. Eicosanoid profiling in the conjunctiva was performed using HPLC-ESI/MS/MS.
Omega-3 fed mice showed a lower clinical score and a decrease in the number of infiltrated eosinophils in the conjunctiva. The expression level of Il4, Il13, Ccl5 and Ccl11 was lower in omega-3 fed mice compared to omega-6 fed mice. The production of inflammatory eicosanoids such as prostaglandin D2 and leukotriene B4 was decreased in the conjunctiva of omega-3 fed mice.
Dietary omega-3 fatty acids might exert therapeutic effects on allergic conjunctivitis by regulating multiple processes including eicosanoid production, eosinophil infiltration and the expression of Th2 cytokines and chemokines .
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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