The Effect of Human Corneal-Derived Mesenchymal Stromal Cells Secretome on the Human Macrophages Viability

Abstract

Purpose : The anti-inflammatory properties of mesenchymal stromal/stem cells (MSCs) have been recognized as one of their major modes of action. Macrophages (Mq) are the key player of the innate immune system in the most stages of inflammation. We evaluated the effect of the human corneal limbal derived- MSCs (CL-MSCs) secretome on the viability of human Mqs.

Methods : CL- MSCs were isolated from healthy cadaver donors and cultured in MEM-alpha media with 10% fetal bovine serum. Their secretome were collected in serum-free media after 48 hours. CD14+ cells were isolated from peripheral blood mononuclear cells and were differentiated into Mqs in IMDM + 10% serum. ApoTox-Glo™ Triplex Assay (Promega) was used to determine viability, cytotoxicity and apoptosis of Mqs after exposure to the CL-MSCs secretome.

Results : There was not a statistical difference in viability and cytotoxicity of Mqs 8 hours after exposure to the CL-MSCs secretome compared to the unconditioned media (P > 0.05 for both). However, there was a 2.4 ± 5.35 folds increase in apoptosis rate of Mqs 8 hours after incubation with CL-MSCs secretome compared to the control (P <0.001).

Conclusions : Inducing apoptosis in the Mqs may be one of the mechanisms that CL-MSCs confer their anti-inflammatory property.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.