June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
In vivo confocal microscopy of corneal wound healing after Descemet’s Stripping Automated Endothelial Keratoplasty (DSAEK)
Author Affiliations & Notes
  • Everardo Hernandez-Quintela
    Cornea Service, Association Para Evitar la Ceguera, Mexico City, Mexico
  • Claudia Ortiz Valencia
    Cornea Service, Association Para Evitar la Ceguera, Mexico City, Mexico
  • Guillermo De Wit
    Cornea Service, Association Para Evitar la Ceguera, Mexico City, Mexico
  • Manuel Ramirez Fernandez
    Cornea Service, Association Para Evitar la Ceguera, Mexico City, Mexico
  • Footnotes
    Commercial Relationships   Everardo Hernandez-Quintela, None; Claudia Ortiz Valencia, None; Guillermo De Wit, None; Manuel Ramirez Fernandez, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1484. doi:
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      Everardo Hernandez-Quintela, Claudia Ortiz Valencia, Guillermo De Wit, Manuel Ramirez Fernandez; In vivo confocal microscopy of corneal wound healing after Descemet’s Stripping Automated Endothelial Keratoplasty (DSAEK). Invest. Ophthalmol. Vis. Sci. 2017;58(8):1484.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Descemet’s Stripping Automated Endothelial Keratoplasty (DSAEK) in vivo wound healing process has not been fully described. To evaluate corneal wound healing findings after DSEAK by confocal microscopy.

Methods : A total of 8 eyes (8 patients) were included. In vivo confocal microscopy (Confoscan 4, Fortune Technologies, Italy) was performed from 4 to 6 weeks after DSAEK. Measurements were done from the corneal endothelium to the corneal surface with Navis® software (NIDEK, Multi-Instrument Diagnostic System, Japan).

Results : Donor-receptor interface was found in an average of 114 μm. Corneal stromal folds were observed from 111.1 ± 3.5 μm from the endothelium to 286 ± 94 μm (mean 175 ± 90.5 μm). Keratocytes were activated in the donor tissue from 12 ± 1.4 μm from the endothelium to 105 ± 38.2 μm to the donor-receptor interface (mean 93 ± 36.9 μm).

Conclusions : Donor keratocytes are activated up to 6 weeks after DSAEK. Several corneal folds are present in proximity to the donor-receptor interface after DSAEK. Further evaluation of these findings is justified to determine its clinical significance.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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