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Zhaoxia Huang, Ling Wang, Xiao-Hui Hu, James W Gigantelli, Quan Dong Nguyen, David W Li; Role of the Mab21 Genes in Lens Epithelial Cells.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1704.
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© ARVO (1962-2015); The Authors (2016-present)
The Mab21 gene family have been shown to play very important roles during vertebrate eye development. Yet the functional mechanisms remain to be further studied.
Mab21L1 and Mab21L2 cDNAs were stably transfected into mouse lens epithelial cells, aTN4-1 line. Their expressions were verified by RT-PCR and Western blot analysis. Cell growth rates of various transfected stable lines were determined with MTT assay. Induced apoptosis and possible molecular mechanisms were analyzed with Hoechst staining and Western blot analysis. Cellular senescence was detected with senescence b-Gal assay.
Mab21L1 when expressed in the transfected cells displays resistence against apoptosis induced by okadaic acid. It promotes lens cell differentiation under induction by 100 ng/ml bFGF. At the molecular level, expression of Mab21L1 downregulates expression of the proapoptotic Bak gene but promotes expression of the differentiation-related aB-crystallin expression under treatment by 100 ng/ml bFGF. In contrast, in Mab21L2-transfected cells, bFGF induction causes downregulation of aB-crystallin gene expression.
Mab21L1 and Mab21L2 have differential functions in lens epithelial cells under bFGF induction. (Supported by Research Prevent Blindness, NSFC-81570824, Zhongshan Ophthalmic Center, and Chinese Scholarship Council).
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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