Abstract
Purpose :
To examine if somatic mutations of KRAS, NRAS, and BRAF are associated with lacrimal gland epithelial tumors in Japanese patients.
Methods :
In this study, 7 cases of lacrimal gland epithelial tumors from a single institution were included. There were five cases of pleomorphic adenoma (PA), two cases of adenoid cystic carcinoma (ACC). DNA was extracted from formalin-fixed paraffin-embedded specimens. We amplified the DNA of KRAS exon 2, NRAS exon2, and BRAF exon 15 using PCR and examined each sample for mutation by direct sequencing.
Results :
There was no KRAS, NRAS, or BRAF mutation in any of the cases. We also evaluated KRAS/NRAS exon2 mutation in the epithelial and mesenchymal components of PA, but no mutations were present in either component.
Conclusions :
KRAS/NRAS exon2 and BRAF exon15 mutations were not found in 7 Japanese cases of lacrimal gland epithelial tumors. According to previous reports, no KRAS mutations have been observed in salivary gland ACC, and no BRAF mutations have been observed in other salivary gland tumors. This suggests that the genetic profile of lacrimal gland epithelial tumors may be similar to that of salivary gland tumors.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.