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Meng Shi, Hsin-Hua Liu, Liwei Zhang, Guangyu Li, John G Flanagan, Lu Chen; In vivo imaging and characterization of Schlemm’s canal in response to ocular hypertension. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2075. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
We recently provided the first evidence showing that Prox-1, the master control gene for lymphatic development, is expressed on Schlemm’s canal, a critical structure in the drainage of aqueous humor. This study was designed to investigate the responses of Schlemm’s canal in Prox-1-GFP mice after the induction of ocular hypertension.
Ocular hypertension was induced in Prox-1-GFP mice by circumlimbal suture. Intraocular pressure (IOP) was measured using a non-invasive TonoLab tonometer. Morphological changes in Schlemm’s canal were monitored for 8 weeks by our custom built live imaging system. Additionally, retinal nerve fiber layer (RNFL) thickness was evaluated by optical coherence tomography (OCT), full-field flash electroretinogram (ERG) was recorded with a VERIS system, and whole-mount retinae were immunostained for Brn3a positive retinal ganglion cells (RGCs) at 8 weeks post-procedure.
In response to IOP elevation, we detected a progressive decrease of Schlemm’s canal area with less continuity, indicating a collapse or occlusion of this structure. Moreover, we quantified significant reductions in RNFL thickness (-8.82%), number of RGCs (-17.32%) and ERG amplitudes for all retinal cell classes (photoreceptors, -12.85%; bipolar cells, -12.81%; and RGCs, -23.37%) (P < 0.0001) at 8 weeks post-procedure.
Intravital imaging of Schlemm’s canal in Prox-1-GFP transgenic mice offers a new approach to study Schlemm’s canal in the murine model of ocular hypertension. Further investigation utilizing this model may facilitate the discovery and development of new mechanisms and therapeutic strategies for glaucoma.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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