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Cassie Janell Gould, Colin J Saldanha, Victoria P Connaughton; Developmental endocrine disruption alters adult visually-guided behaviors in the Zebrafish.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2226. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the short- and long-term effects of two anti-estrogenic endocrine disruptors, formestane and PCB1254, on larval visual system anatomy and adult visually-guided behaviors.
Zebrafish larvae were transiently exposed to environmentally-relevant concentrations of each chemical at 24hpf (hours postfertilization), 72hpf, 7d (days) pf, and 21dpf and the acute effects on overall anatomy were determined. At 3-months of age, adult optomotor responses were assessed. To determine the mechanism of action of the endocrine disruptors, aromatase activity was measured using adult zebrafish brain tissue.
There were no significant anatomical differences nor an effect on aromatase activity (One Way ANOVA, p=0.86) as a result of acute exposure to PCB1254. In contrast, acute development exposure to formestane potently inhibited aromatase activity (One way ANOVA, p<0.01) and significantly diminished adult visually guided behaviors, as there was a main effect of treatment between the control stimulus ON and treatment stimulus ON groups (2x2x2 ANOVA, p<0.01). Interestingly, however, there were no significant gross morphological differences as a result of formestane exposure.
Inhibition of aromatase activity by formestane during specific developmental time points results in diminished visually-guided responses in adults suggesting long-term consequences.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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