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Tae-eun Lee, Yong Yeon Kim; Effect of Doxycycline on Retinal Ganglion Cells in an Experimental Glaucoma Model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2572. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effect of doxycycline on the death of retinal ganglion cells (RGCs) and impacting matrix metalloproteinase (MMP) expression in an experimental model of glaucoma.
The glaucomatous experimental model was induced by cauterizing vortex and episcleral veins in the eyes of 36 rabbits. The effects of oral doxycycline administration at dosages of 10 mg/kg/day (n=12) and 30 mg/kg/day (n=12) were evaluated and compared with the untreated controls (n=12). The eyes were histologically examined for RGC death at two and four weeks. The expression and activity of MMP-2 and MMP-9 were measured by immunohistochemical analysis and gelatin zymography.
When compared to the baseline value the intraocular pressure of the operated eyes was significantly elevated at each time point. Significantly denser RGCs were found in the retinal ganglion cell layer and lower rates of apoptosis were observed in the high dose treatment (30 mg/kg/day) group, as compared to the control group at two and four weeks after treatment, whereas in the low dosetreatment (10 mg/kg/day) group, such change was observed only at two weeks post-treatment. The expressions and the gelatinolytic activity of MMP-2 and MMP-9 were significantly suppressed in the doxycycline treatment group at two and four weeks.
The result of this study suggests that doxycycline could contribute to delaying the death of RGCs through the down-regulation of MMPs, at least in part, in this experimental model of glaucoma.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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