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Justin Christopher Christopher D'Antin, Rafael I Barraquer, Ralph Michael; Prevention of posterior capsule opacification in cultured human lens capsules, through treatment with hydrogen peroxide or distilled water.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3194.
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© ARVO (1962-2015); The Authors (2016-present)
The high rates of posterior capsule opacification (PCO) after cataract surgery continue to be a problem for many patients worldwide. We tested the hypothesis that PCO could be delayed or inhibited through the application of hydrogen peroxide (H2O2) or distilled water (H2Od) during cataract surgery. Using cultured human lens capsules as an experimental model.
Lens capsule-ciliary body complexes were extracted from human donor eyes (n = 22, ages 43-89). Samples were treated for 5 min with either 30mM H2O2 (n=8) or H2Od (n=7) or used as untreated controls (n=7). Treatment was applied after lens extraction through intercapsular irrigation using a silicone irrigation ring. Samples were cultured on average for 30 days, during which dark field and lateral illumination photos were taken every 2 to 3 days. These photos were used to observe and quantify, time until cellular growth and subsequent confluence on the posterior capsule. Growth prevention was defined as the absence of cells on the posterior capsule within the visual limits of the anterior rhexis. Kaplan Meier survival analysis was used to interpret the data.
One control and two H2Od samples were contaminated before useful results could be obtained so they were discarded. Growth prevention on day 10 was 100% for both treatments and 0% for controls. Estimated growth prevention on day 20 was 100% for H2O2 and 50% H2Od and on day 30 it was 58% for H2O2 and 50% H2Od. The overall prevention of cell growth compared to control was significant for both treatments (H2Od p=0.001 and H2O2 p<0.001) while there was no significant difference between treatments. Until day 28 none of the treated samples that had shown growth reached confluence. At day 30, 25% of H2Od and 15% of H2O2 samples reached confluence. Confluence for controls was 50% on day 10 and 83% on day 20, reaching 100% on day 26.
The results are consistent with our hypothesis that cell growth and PCO can be prevented or significantly delayed by applying hydrogen peroxide (H2O2) or distilled water (H2Od). Although, some cell growth and PCO was observed in treated samples, it appeared significantly later than in controls and after the end point (day 28) of most other studies.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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