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Nosaibah Hariri, Vivian Snyder, Faris Hashem, Carla Carrilho, Peter J Kobalka, Wilson O Lin, Yu-Tsueng Liu, Eliane Samo Gudo, Evelia Marole, Yolanda Zambujo, Robert T Schooley, Jonathan Lin; The Association Between Ocular Surface Squamous Neoplasia and Human Papilloma Virus in HIV Sero-positive Mozambicans. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3348.
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© ARVO (1962-2015); The Authors (2016-present)
The role of Human papilloma virus (HPV) in ocular surface squamous neoplasia (OSSN) from HIV sero-positive (HSP) patients is controversial. Our hypothesis was that OSSN in HSP patients would be driven by HPV and thus would demonstrate p16 expression, a well-established biomarker of HPV-associated squamous neoplasms. We studied the hypothesis by retrospectively surveying OSSN from a large HIV sero-positive cohort from Mozambique.
A total of 75 patients from Mozambique were selected. Forty-seven were female and 28 were male. The age ranged from 21 to 70. Formalin fixed, paraffin-embedded tissue blocks from biopsies and resections were cut and stained with hematoxylin and eosin (H&E). We reviewed the H&E slides and assigned diagnoses. All cases were evaluated with p16 immunohistochemistry. Cases were classified as positive when the neoplastic cells showed strong nuclear and cytoplasmic expression. These cases were further subdivided by HIV status, diagnosis, gender, and age and analyzed for trends.
Conjunctival intraepithelial neoplasia (CIN) was the diagnosis in 22 (29%) patients, and 51 (68%) were diagnosed with invasive squamous cell carcinoma (SCC). The remaining 2 (3%) cases had benign diagnoses. Sixty-three (84%) patients were HIV-positive and 12 (16%) were HIV-negative. Sixty-one (81%) cases were negative for p16, and 14 (19%) were positive. Of the HIV-positive cases, 19% were p16 positive; 17% of the HIV-negative cases were p16 positive. Only 5% of the CIN cases were p16 positive, while 34% of the SCC cases were positive.
Contrary to other studies, we found no association between p16 expression and OSSN in our Mozambican cohort. Additionally, there was no statistically significant difference between p16 reactivity in HIV-positive and negative patients (19% and 17%, respectively).Interestingly, there was a significant difference in p16 positive CIN cases (5%) and p16 positive SCC cases (34%).
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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