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Laurenz L Sonnentag, Anna Kraus, Mahdy Ranjbar, Julia Lueke, Salvatore Grisanti, Aysegul Tura; Outcomes of quercetin treatment on the metastatic profile of a uveal melanoma cell line. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3966.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the efficacy of the dietary flavonoid quercetin on suppressing the viability, proliferation, and migration of the uveal melanoma cells as well as the expression of major proteins involved in metastatic activity.
Cultures of 92.1 cells were incubated with quercetin at a concentration range of 1-100 µM. Viability was analysed by the MTT-test, Live-Dead staining, and TUNEL-assay. Cell proliferation was assessed by BrdU incorporation and Ki-67 immunostaining. Migration was analyzed by the wound healing assay. Protein expression was analysed by immunocytochemistry and -blotting.
Quercetin resulted in a dose-dependent reduction in cell proliferation, survival, and migration, with the effects becoming significant (P<0.05) from 25 µM onwards. Quercetin also interfered with the expression of the insulin-like growth factor-1 receptor on the cell surface, possibly by preventing the upregulation of the transcriptional coactivator YAP-1 in the nucleus.
These findings demonstrate the efficacy of quercetin as a novel therapy alternative for suppressing the metastatic potential of uveal melanoma cells.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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