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Bryan Yue, Cecilia S Lee, Sundeep Kasi, Bryan Hong, Lakshmi Akileswaran, Doug Baughman, Aaron Y Lee, Sunir Garg, Russell N Van Gelder; Molecular Diagnostic Evaluation of Post-surgical Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4255. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To characterize the viral pathogens found in post-surgical endophthalmitis with whole genome sequencing (WGS) and polymerase chain reaction (PCR).
Patients diagnosed with endophthalmitis following intraocular procedures were recruited from Wills Eye Hospital and University of Washington from Sept 1st , 2014 to Dec 31st , 2015. All patients underwent vitreous or aqueous tap. The specimens were sent for microbial culture and additional PCR and WGS. Following DNA extraction, quantitative PCR for Torque Teno Virus (TTV), Merkel Cell Polyomavirus (MCV), and actin were performed in all samples. WGS results were analyzed by Scalable Metagenomics Alignment Research Tool (SMART). PCR results were verified via gel electrophoresis.
A total of 30 samples were collected (12 culture-positive (40%), 14 culture-negative (47%), 4 unknown). WGS confirmed 100% of the bacterial culture results (12/12) and revealed substantial amounts of Staphylococcus in 2 culture-negative samples. In addition, the WGS results allowed de novo genomic reconstruction of pathogens in several samples with high specificity. Overall, 20 (66.66%) samples were positive for TTV and 15 (50%) for MCV. The presence of TTV or MCV was not associated with culture positivity by Fisher’s exact test.
Viral DNA sequences are unexpectedly common in endophthalmitis. The role of these viruses in this disease process remains unclear. WGS and de novo reconstruction of pathogen’s genome may become a useful technique in pathogen detection in endophthalmitis.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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