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Isaak Fischinger, Theo Gunther Seiler, Beatrice E Frueh, Matthias Miller; Biomechanical properties after corneal crosslinking using riboflavin in dextran solution or in hydroxy propyl methyl cellulose. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4315.
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© ARVO (1962-2015); The Authors (2016-present)
To compare corneal stiffening in porcine eyes induced by UV-crosslinking using riboflavin solved in 20% dextran solution and hydroxy propyl methyl cellulose (HPMC).
Three groups of seven corneas of freshly enucleated porcine eyes were compared regarding their biomechanical properties. Crosslinking with an UVA-irradiance of 9mW/cm2 for 10 minutes was performed on corneas of group one and two using the UVX 2000 lamp (IROC Innocross, Zürich, Switzerland). Group one was treated with 0.1% riboflavin in 20% dextran solution, group two with isotonic solution containing 0.1% riboflavin and 1.0% HPMC and group three served as control group. Stress-strain measurements were performed on 5mm wide corneal strips at strains up to 12% using a material tester (UStrech, Cellscale, Ontario).
The stress needed for a strain of 12% was increased significantly in corneas of group one (by 56%) and group two (by 123%) compared to the control group three (p≤0.01 for both groups). Between group one and two there was no significant difference regarding the stress-strain measurements (p=0.064), though there was a clear tendency showing more stiffening in group one using the dextran solutions.
These experiments indicate that the biomechanical stiffening of crosslinking using dextran and HPMC is comparable, however there seems to be a slightly stronger stiffening effect using dextran as a riboflavin carrier which was not statistical significant in this small cohort.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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