June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Organotypic epithelium tissue model testing reveals high-concentration tear fluid lysozyme as a potential inducer of conjunctiva inflammation
Author Affiliations & Notes
  • Hiroyuki Nawase
    Singapore Institute for Clinical Sciences, Singapore, Singapore
    SEED Co.,LTD., Saitama, Japan
  • Takao Sato
    SEED Co.,LTD., Saitama, Japan
  • Nobuyo Yawata
    Singapore Eye Research Institute, Singapore, Singapore
    Duke-NUS Graduate Medical School, Singapore, Singapore
  • Makoto Yawata
    Singapore Institute for Clinical Sciences, Singapore, Singapore
    National University of Singapore, Singapore, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Hiroyuki Nawase, SEED Co.,LTD. (E); Takao Sato, SEED Co.,LTD. (E); Nobuyo Yawata, None; Makoto Yawata, SEED Co.,LTD. (F)
  • Footnotes
    Support  IAF111126, Agency for Science, Technology and Research (A*STAR)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4358. doi:
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    • Get Citation

      Hiroyuki Nawase, Takao Sato, Nobuyo Yawata, Makoto Yawata; Organotypic epithelium tissue model testing reveals high-concentration tear fluid lysozyme as a potential inducer of conjunctiva inflammation. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4358.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : Inflammation of the conjunctiva surface can be triggered by deposition of protein constituents of tear fluid onto contact lenses, primarily lysozyme, lactoferrin and albumin, and this can result in formation of giant papillary conjunctivitis. In this study we utilized an organotypic conjunctiva epithelium model to test for the effects of these proteins on conjunctiva epithelium.

Methods : Tissue models resembling the conjunctiva epithelium were constructed by first generating a fibroblast scaffold and then layering on primary conjunctiva epithelial cells. Using the air-lift culture technique, the epithelium is stratified to resemble the human conjunctiva. Human lysozyme, lactoferrin and albumin were applied individually to the model at different concentrations. As readout, the levels of 27 soluble factors including inflammatory cytokines and chemokines were quantified in the supernatants.

Results : The levels of IL-1β, b-FGF, IP-10, MIP-1α and MIP-1β were significantly up-regulated after stimulation with high concentration of lysozyme. The concentration was comparable to the level of protein deposition on contact lenses. High concentration-lactoferrin also increased MIP-1α and MIP-1β expression, although the increases were modest compared to that of lysozyme. Albumin did not induce such changes in inflammatory factors.

Conclusions : The results indicate that high concentration of lysozyme comparable to the levels found in the deposition can increase expression of various inflammatory factors as compared to lactoferrin and albumin. The profile of inflammatory factors that are produced by the organotypic conjunctiva tissue model in response to lysozyme implies that lysozyme can trigger an inflammatory process in the epithelium at high-concentrations.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.


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