The effect of enhancing uptake of drug by Fusarium Solani using micelles as a vehicle

Abstract

Purpose :
To study the penetration of coumarin-6 （C-6） into Fusarium solani using micelles as a vehicle.

Methods : Fungal spores were collected after the cultured on potato-dextrose agar. C-6 was used a hydrophobic model drug and was entrapped into micelles by using solvent evaporation method （C-6-M）. C-6 nanoparticle was prepared by recrystallization and used as a control (C-6-N). Both C-6-M and C-6-N were diluted using saline to 0.3%. C-6-M was added into the mixture of spores suspension and sugar-salt medium (7×10⁶ spores/ml) as an experimental group. C-6-N was added the same concentration of spores’ mixture as a control group. Both experimental and control groups were incubated under 37°C. The mixtures were taken at 0, 1, 6, 12 and 24 hours respectively, and observed under the confocal microscopy and recorded the pictures. The photos were conducted using imaris software. The fluorescent intensity was calculated in unit volume (FI/μm³). The data were compared by t-test at corresponding different time points.

Results : Fluorescence was appeared in the interior of spores or hyphae. The fluorescent intensity in the C-6-M (experimental group) was significantly higher than C-6-N (group) at different time points except at 0 and 24 h

Conclusions : Micelles as a vehicle significantly enhanced the hydrophobic model drug coumarin-6 to penetrate into the interior of Fusarium solani.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.