June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Repeatability and reproducibility of foveal avascular zone area measurement on normal eyes by different optical coherence tomography angiography instruments.
Author Affiliations & Notes
  • Anna Crepaldi
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Elisabetta Pilotto
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Enrico Della Dora
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Davide Deganello
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Luisa Frizziero
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Enrica Convento
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Evelyn Longhin
    Department of Ophthalmology, University of Padova, Padova, Italy
  • Edoardo Midena
    Department of Ophthalmology, University of Padova, Padova, Italy
    G.B. Bietti Foundation, IRCCS, Rome, Italy
  • Footnotes
    Commercial Relationships   Anna Crepaldi, None; Elisabetta Pilotto, None; Enrico Della Dora, None; Davide Deganello, None; Luisa Frizziero, None; Enrica Convento, None; Evelyn Longhin, None; Edoardo Midena, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4847. doi:
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      Anna Crepaldi, Elisabetta Pilotto, Enrico Della Dora, Davide Deganello, Luisa Frizziero, Enrica Convento, Evelyn Longhin, Edoardo Midena; Repeatability and reproducibility of foveal avascular zone area measurement on normal eyes by different optical coherence tomography angiography instruments.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4847.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare foveal avascular zone (FAZ) area measurements produced by different optical coherence tomography angiography (OCTA) devices when imaging normal eyes.

Methods : All eyes in this cross-sectional study underwent OCTA using two different OCTA devices: Spectralis HRA+OCTA (Heidelberg Engineering) and Nidek RS-3000 Advance device (Nidek, Gamagori, Japan). Using OCT en face angiograms, automatically segmented into the superficial retinal capillary plexus (SCP) and the deep retinal capillary plexus (DCP), two graders manually measured the FAZ area.

Results : A total of 59 normal eyes from 33 subjects were included (17 males, 16 females; mean 26 ± 3 years of age).
Mean FAZ areas were 0.327 ± 0.091 mm2 at the SCP and 0.568 ± 0.173 mm2 at the DCP measured at Nidek RS 3000, 0.299 ± 0.085 mm2 at the SCP and 0.349 ± 0.079 mm2 at the DCP measured at Spectralis. The two devices’ measures were significantly different both at the SCP and at the DCP (p< 0.0001, for both).
The intra-operator agreement was excellent with both devices at the SCP [ICC= 0.96 (95% CI: 0.93 to 0.98) with Nidek RS-3000 and 0.97 (95% CI: 0.93 to 0.98) with Spectralis]. At the DCP, it was good with Spectralis [ICC: 0.85 (95% CI: 0.62 to 0.93), fair with Nidek RS-3000 [ICC= 0.64 (95% CI: 0.33 to 0.81)].
The inter-operator agreement was excellent for Spectralis [ICC= 0.97 (95% CI: 0.93 to 0.98), good for Nidek RS-3000 [ICC= 0.93 (95% CI: 0.57 to 0.98) at the SCP. It was good at the DCP with both devices [ICC: 0.74 (95% CI: 0.52 to 0.87) with Nidek RS3000; and 0.81 (95% CI: 0.63 to 0.91) with Spectralis].

Conclusions : OCTA is a reliable method for the quantification of FAZ area, particularly when measured in the SCP. However, FAZ area measurements obtained with different OCTA devices are different. These differences appear to be primarily attributable to the analysis algorithms used to set SCP and DCP. These findings should be taken in consideration when planning a follow-up of patients with retinal vascular diseases that affect capillary plexuses.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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