June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017


Correlations between cytokine levels in aqueous humor, disease duration and intraocular pressure in acute angle closure glaucoma attack
Author Affiliations & Notes
  • Tomohito Sato
    Ophthalmology, National Defense Medical College, Tokorozawa, Japan
  • Masaru Takeuchi
    Ophthalmology, National Defense Medical College, Tokorozawa, Japan
  • Yoko Karasawa
    Ophthalmology, National Defense Medical College, Tokorozawa, Japan
  • Takayuki Kanda
    Ophthalmology, National Defense Medical College, Tokorozawa, Japan
  • Masataka Ito
    Developmental Anatomy and Regenerative Biology, National Defense Medical College, Tokorozawa, Japan
  • Toshio Enoki
    ENOKI EYE CLINIC, Sayama, Japan
  • Footnotes
    Commercial Relationships   Tomohito Sato, None; Masaru Takeuchi, None; Yoko Karasawa, None; Takayuki Kanda, None; Masataka Ito, None; Toshio Enoki, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4899. doi:
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      Tomohito Sato, Masaru Takeuchi, Yoko Karasawa, Takayuki Kanda, Masataka Ito, Toshio Enoki;

      Correlations between cytokine levels in aqueous humor, disease duration and intraocular pressure in acute angle closure glaucoma attack. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4899.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To identify cytokines in aqueous humor related to disease duration and intraocular pressure (IOP) before and after phacoemulsification in patients with first acute angle closure glaucoma attack.

Methods :
Eleven eyes of 10 patients, in whom phacoemulsification was performed to resolve first acute angle closure glaucoma attack after conservative treatments such as IOP-reducing drops, internal drugs and intravenous drips, were enrolled (males / females ; 4 / 6, average age ± standard deviation ; 73.0 ± 8.64 years old). Undiluted aqueous humor samples were collected at the initiation of the surgery. The levels of VEGF, IL-6, IL-8 and G-CSF in the samples were measured by a multiplex bead array. Correlations between these cytokines and IOP at initial visit (43.7 ± 12.1 mmHg), IOP just before the surgery (33.2 ± 18.8 mmHg), IOP one week later after the surgery (17.0 ± 9.46 mmHg), IOP one month later after the surgery (16.9 ± 6.93 mmHg) and disease duration from awareness to the surgery (11.9 ± 16.3 days) were analyzed using Spearman's correlation. P value less than 0.05 was considered significant.

Results :
There was no significant difference between IOP at initial visit and all kinds of cytokines. Positive relations were shown between IOP just before the surgery and levels of IL-8 and G-CSF. There were negative correlation between IOP at one week after the surgery and level of G-CSF, and between IOP at one month after the surgery and level of IL-8 and G-CSF, respectively. In addition, negative correlations were shown between disease duration and levels of VEGF, IL-8, and G-CSF.

Conclusions :
In eyes with first acute angle closure glaucoma attack, persist high IOP could accelerate cell proliferation and infiltration of inflammatory cells. Elimination of cytokines in the aqueous humor by phacoemulsification would contribute to lowering IOP effect by the surgery.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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