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takashi nishida, Yoshiki Kuse, Kiyofumi Mochizuki, Tetsuya Yamamoto, Masamitsu Shimazawa, Hideaki Hara; Low concentrations of levofloxacin can decrease UV-induced cellular damage. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5779. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Fluoroquinolones are widely used to treat patients with various kinds of infections. They, however, have adverse effects such as uveitis and retinal detachment. The purpose of this study was to determine whether therapeutic levels of fluoroquinolones can decrease the ultraviolet (UV) light damage of cultured ocular cells.
Levofloxacin was selected as the fluoroquinolone to test the effects of UV light-induced cell damage. Levofloxacin was added to cultures of primary human retinal pigment epithelial (hRPE) cells, primary retinal cells, and human adult retinal pigment epithelial cell line (ARPE-19). The viability of the hRPE cells was assessed by the Cell Counting Kit-8. The cellular damage induced by UV light exposure was analyzed by immunostaining of S-opsin and cleaved caspase-3 in primary retinal cells. The number of S-opsin-positive and cleaved caspase-3-positive cells were counted. The level of AKT and CREB were determined by Western blot analysis of ARPE-19 cells.
UV exposure affected the cell viability, cell death, protein expression (phosphorylated Akt and CREB) in cultured ocular cells. HRPE cells exposed to low doses of levofloxacin increased the cell viability that was reduced by UV exposure. Primary retinal cells were protected against UV light-induced cone photoreceptor cell death by exposure to levofloxacin. UV exposure altered the phosphorylated level of Akt and CREB, and levofloxacin did not increase the level of expression (P = 0.753 and P = 0.615, respectively, Two-way ANOVA).
These results demonstrated that the UV-induced damage of cultured cells can be decreased by low concentrations of levofloxacin. The mechanism for this may be through the depression of oxidative stress.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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