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Namrata Nandakumar, Francisco J Lopez, Gianna C Teague, Kameran Lashkari; Analysis of Vitreous Biomarkers in Proliferative Diabetic Retinopathy and Macular Edema. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5807.
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We have previously identified various pro-angiogenic and pro-inflammatory biomarkers in the vitreous of subject with proliferative diabetic retinopathy (PDR). In this study, we have performed a more detailed subgroup analysis of subject with PDR with and without diabetic macular edema (DME), cystoid macular edema (CME), and non-diabetic control.
Vitreous samples were collected from subjects with PDR (n=65), CME (n=30 ) and control (n=35) were studied by multiplex analysis (Bio-Rad Analyzer) of 31 pro-angiogenic and pro-inflammatory factors. The PDR group was further analyzed for presence (n=35) and absence of DME (n=30). Data was transformed to allow for parametric analysis including MANCOVA analysis.
There were no differences in age or gender between the PDR with DME, PDR without DME, non-diabetic CME and control groups. MANCOVA analysis of 31 factors showed changes in profile of pro-angiogenic and pro-inflammatory factorsamong the PDR-no DME; PDR-DME and CME groups. PLGF, VEGF-A, angiopoietin, IL-8, TNFa and leptin concentrations were higher in both PDR with or without DME (p<0.05). This pattern was distinct from the pattern observed in CME and control groups.
The changes in vitreous cytokines and other factors in PDR may reflect their participation in the disease process. Overall, MANCOVA analysis identified 6 distinct factors that were significantly altered in PDR with/without DME. Further studies will elucidate biomarkers specifically associated with DME.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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