Cells were incubated for 10 minutes on ice with 20 μg/mL TruStain fcX 93 (Biolegend, San Diego, CA, USA) for nonspecific binding. Subsequently, cells were stained for 30 minutes and placed on ice with combinations of the following antibodies: (1) for myeloid cells: FITC-Ly6C, HK1.4 (Biolegend); PE-Ly6G, 1A8; PECy7-CD11c, HL3; PE-CF594-CD11b, M1/70; Alexa Fluor 700-CD45, 30-F11; V500-CD3e, 500A2; BV-421-NK1.1, PK136 (BD Bioscience, San Jose, CA, USA); APC-F4/80, BM8; PerCP-eFluor710-CD115, AFS98 (eBioscience, San Diego, CA, USA); (2) for progenitor cells: FITC-CD117, 2B8 (eBioscience); PE-CD34, MEC14.7; PECy7-Ly6A/E, D7; BV-421-Lineage cocktail (Biolegend); Alexa Fluor 700-CD45, 30-F11 (BD Bioscience). Samples were washed once with FACS buffer, then with PBS, stained for 30 minutes, and placed on ice with APCefluor780-fixable/viability dye (eBioscience) for live/dead cell discrimination. After two more washes, cells were fixed with 1% formalin and acquired on a LSR Fortessa (BD Bioscience) flow cytometer (at the flow cytometry facility of Indiana University School of Medicine, Indianapolis, IN, USA). All cells (events) inside each tube were acquired for each sample to represent the total amount of cells per sample. CD45hi and microglia cell numbers represent the total events acquired by flow cytometry in their respective gates of each sample. Data were analyzed using FlowJo 10.2 software (FlowJo, Ashland, OR, USA).