For cell surface markers, cells were stained in PBS containing 2% fetal bovine serum for 15 minutes, unless mentioned otherwise. For intracellular cytokine staining, cells were stimulated with phorbol 12-myristate acetate (PMA) (50 ng/mL) and ionomycin (1 μg/mL), or with different antigens, the last 8 hours with 10 μg/mL Brefeldin A and/or 2 μmol/mL monensin, at 37°C and 5% CO2 as mentioned above, and then fixed and stained according to manufacturer's protocols. Antibodies used were as follows: CD3-Alexa Fluor 700, CD4-PerCP/Cy5.5A, IFN-γ Alexa Fluor 488, IL-17–PerCP/Cy5.5A, CCR7-APCeFluor780 (eBioscience, San Diego, CA, USA), CD8-V500A (Tonbo, San Diego, CA, USA), TNF-α–Alexa Fluor 700(BD), CD45RA-PECy7, CD45RO-PECF594, FAS-PECF594 (BD, San Diego, CA, USA), FasL-PE (Invitrogen), AnnexinV-APC (Immuno Tools, GmbH, Germany). Cells were acquired and analyzed by BD LSR Fortessa II (BD), using FlowJo (Ashland, OR, USA) three-star or FACSDIVA 6 software (BD Biosciences, San Jose, CA, USA).