The prescreening test showed several substances, which showed no effect in our model. As those are all drugs, which have been suggested for the prevention of PCO in peer reviewed manuscripts, we compared those articles with our work, and why we could not reproduce this effect. Some of the substances were already proven ineffective in other studies, for example, heparin,
36 prednisolone,
37 dexamethasone, and diclofenac.
38 Some other substance were used at higher concentration in order to cause toxic lens epithelial cell death, for example, trypan blue,
39 lidocaine,
40 or EDTA.
41 By inclusion criteria, we excluded toxic effects causing cell death in our work. Furthermore, authors have applied different IOL drug-loading principles.
42 In addition, some of the proposing authors have used different models, for example, different species.
43 Current data demonstrate that the human capsular bag model is effective for PCO research, and might be superior to other nonhuman models.
7 This applies to basic research on PCO pathogenesis
44–46 as well as to relevant clinical questions, such as the evaluation of novel IOL technology
47 or the feasibility of PCO prophylaxis.
13 For pharmacologic approaches to PCO prevention in particular, there is a need for human models because animal models might differ in regulation mechanisms like signal transduction pathways and growth factor secretion.
7