After imaging on the day of peak inflammation, animals were euthanized and samples collected for flow analysis. Prior to collection, each eye was washed with 1× PBS and dried with a Kimwipe (Kimberly-Clark Professional, Roswell, GA, USA). Corneal paracentesis was performed using a 30-gauge needle (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), and aqueous humor collected from the ocular surface using a capillary tube (Sarstedt, Nümbrecht, Germany). Aqueous was transferred to an Eppendorf tube containing 90 μL cell collection buffer (1× PBS, 1× protease inhibitor [Sigma-Aldrich Corp., St. Louis, MO, USA], and 0.1% bovine serum albumin [BSA] [Fisher Scientific, Waltham, MA, USA]). Approximately 10 to 15 μL aqueous was collected from each eye. The eye was then enucleated and placed in a petri dish (Fisher Scientific). The cornea was removed at the limbus using Vannas scissors (World Precision Instruments, Sarasota, FL, USA), and the lens and vitreous removed together from the remaining eye cup. The vitreous (approximately 20 μL) was manually separated from the posterior lens and placed in a tube containing 90 μL cell collection buffer. Samples were stored on ice after collection and prior to staining. All microsurgical procedures were performed using a Leica M60 stereomicroscope (Leica Biosystems, Inc., Buffalo Grove, IL, USA) with coaxial illumination Leica KL1600 LED (Leica Biosystems, Inc.). Cell counting was performed on a Nexelcom Cellometer Auto 2000 Cell Viability counter (Nexelcom Bioscience, Lawrence, MA, USA) with acridine orange/propidium iodide (AO/PI) staining solution for live/dead mammalian nucleated cells (Nexelcom Bioscience). Cell number is reported as live cells/mL. No chemical or mechanical tissue disruption of the samples was performed prior to flow analysis.