July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Changes in hyper-dry amniotic membrane after implantation in the rabbit conjunctiva
Author Affiliations & Notes
  • Akio Miyakoshi
    Ophthalmology, University of Toyama, Toyama, Japan
  • Atsushi Hayashi
    Ophthalmology, University of Toyama, Toyama, Japan
  • Yasuhiko Nishida
    AMNOS Company, Tokyo, Japan
  • Atsushi Tanaka
    AMNOS Company, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Akio Miyakoshi, None; Atsushi Hayashi, None; Yasuhiko Nishida, None; Atsushi Tanaka, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 153. doi:
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    • Get Citation

      Akio Miyakoshi, Atsushi Hayashi, Yasuhiko Nishida, Atsushi Tanaka; Changes in hyper-dry amniotic membrane after implantation in the rabbit conjunctiva. Invest. Ophthalmol. Vis. Sci. 2018;59(9):153.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To examine histologic changes in hyper-dry amniotic membrane (HDAM) after an implantation surgery in the conjunctiva of rabbit and to compare them to those of Ambio2TM (IOP ophthalmics, Costa Mesa CA).

Methods : Twenty seven rabbits were used and divided into 2 groups: HDAM group (36 eyes of 18 rabbits) and Ambio2 group (18 eyes of 9 rabbits). All surgeries were performed under aseptic conditions and pursuant to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Under general anesthesia, the conjunctiva was cut 6mm by 4mm along the temporal limbus in each eye and the sclera was exposed. HDAM or Ambio2 of 5 mm by 3 mm sheet was sutured on the sclera with 10-0 nylon and covered with the conjunctival autograft. Corticosteroid and antibiotics drops were continued 3 times daily for 1 month. For histological analysis, the state of amniotic membrane, foreign body granulomas, and cellular infiltration, were evaluated in hematoxylin/eosin-stained sections of each eye at 1, 3, and 6 months after surgery.

Results : In the both groups the amniotic membrane was observed in the eyes of 100%, 17%, and 0% at 1, 3, and 6 months after surgery, respectively. In the HDAM group the foreign body granulomas were observed in the eyes of 75%, 17%, and 0% at 1, 3, and 6 months after surgery, respectively, whereas in the Ambio2 group they were observed in the eyes of 67%, 17%, and 0%, respectively. In the HDAM group infiltration of inflammatory cells was observed in the eyes of 100%, 58%, and 8% at 1, 3, and 6 months after surgery, respectively, whereas in the Ambio2 group they were observed in the eyes of 67%, 67%, and 17%, respectively.

Conclusions : Both of HDAM and Ambio2 were completely absorbed without scarring in 6 months after surgery. They showed histologically equivalent responses.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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