July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Effects of multiple intravitreal anti-VEGF injections in a rabbit model of persistent retinal neovascularization (PRNV)
Author Affiliations & Notes
  • Yong Li
    Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Joanna Marie Busoy
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Ben Alfyan Achirn Zaman
    Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore
  • Jay Ji-Ye Wei
    Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Gavin Tan
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Ning Cheung
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Veluchamy A Barathi
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Walter Hunziker
    Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore
  • Wanjin Hong
    Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore
  • Tien Yin Wong
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
    Duke NUS Medical School, Singapore, Singapore
  • Chui Ming Gemmy Cheung
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Yong Li, None; Joanna Marie Busoy, None; Ben Alfyan Achirn Zaman, None; Jay Ji-Ye Wei, None; Gavin Tan, None; Ning Cheung, None; Veluchamy Barathi, None; Walter Hunziker, None; Wanjin Hong, None; Tien Yin Wong, None; Chui Ming Gemmy Cheung, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 195. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yong Li, Joanna Marie Busoy, Ben Alfyan Achirn Zaman, Jay Ji-Ye Wei, Gavin Tan, Ning Cheung, Veluchamy A Barathi, Walter Hunziker, Wanjin Hong, Tien Yin Wong, Chui Ming Gemmy Cheung; Effects of multiple intravitreal anti-VEGF injections in a rabbit model of persistent retinal neovascularization (PRNV). Invest. Ophthalmol. Vis. Sci. 2018;59(9):195.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Multiple intravitreal injections of anti-VEGF agents that are commonly needed for the treatment of intraocular vascular diseases, however, studies have yet to compare the long-term effectiveness and safety of repeated intravitreal injections with these agents.
The purpose of this study is to develop an experimental model of retinal neovascularization with persistent leakage in rabbits to evaluate the long term effect of the repeated intravitreal injections of the anti-VEGF drugs currently in clinical use.

Methods : Persistent retinal neovascularization (PRNV) was induced by a single intravitreal injection of gliotoxin, DL-α-aminoadipic acid (DL-AAA) in Dutch-Belted rabbits. Pharmacology study was performed after 8 weeks of the PRNV induction with stable PRNV-related fluorescein leakage. Three anti-VEGF agents that currently use in clinical were formatted 30ul, equal to 0.6 times human dose and masked with Drug A, B and C. The 3 agents were administrated by intravitreal injections for 3 times and repeated in every 9 weeks.
The effects of multiple intravitreal anti-VEGF injections was evaluated by non-invasive ocular imaging including fundus Photography, fluorescein angiography (FA) and optical coherence tomography (OCT) and were followed up for a total of 36 weeks. The eyes were collected for histological and immunohistochemical analysis at the endpoint.

Results : DL-AAA injection produced clinical PRNV as revealed by fluorescein angiography (FA) at 8 weeks with retinal disorganization in the visual streak. The hyper-fluorescent leaking lesion increased rapidly in the first 4 weeks, stabilized during 4 to 8 weeks, and was persistent throughout the entire observation of 12 months without regression. Intravitreal administration of the anti-VEGF drugs caused complete inhibition of the hyper-fluorescent leak for about 6 to 8 weeks for the all 3 agents. However, there was a difference in the efficacy between drugs seen after the 3 repeat injections. Drug A demonstrated great reduction of leakage as well regression of total PRNV area compared with the other anti-VEGF agents. It was confirmed by immunohistochemistry indicated the beneficial effects on PRNV regression.

Conclusions : This model provides a unique tool to evaluate novel anti-VEGF formulations and therapies and in particular, to compare their duration of action.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×