July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
A Surgical Technique for Subretinal Delivery of Human iPSC-RPE Patch
Author Affiliations & Notes
  • Juan Amaral
    Stem cell and translational research unit, National Eye Institute, Bethesda, Maryland, United States
  • Steve T Charles
    Charles Retina Institute, Memphis, Tennessee, United States
  • Vladimir Kristov
    Section on Epithelial and Retinal Phisiology and Disease, National Eye Institute, Bethesda, Maryland, United States
  • Arvydas Maminishkis
    Section on Epithelial and Retinal Phisiology and Disease, National Eye Institute, Bethesda, Maryland, United States
  • Kapil Bharti
    Stem cell and translational research unit, National Eye Institute, Bethesda, Maryland, United States
  • Footnotes
    Commercial Relationships   Juan Amaral, None; Steve Charles, None; Vladimir Kristov, None; Arvydas Maminishkis, None; Kapil Bharti, None
  • Footnotes
    Support  IRP NIH & Common fund NIH
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 68. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Juan Amaral, Steve T Charles, Vladimir Kristov, Arvydas Maminishkis, Kapil Bharti; A Surgical Technique for Subretinal Delivery of Human iPSC-RPE Patch. Invest. Ophthalmol. Vis. Sci. 2018;59(9):68.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Success of a cell therapy for AMD requires safe delivery of iPSC-RPE-patch using a surgical technique that is simple and reproducible. We have developed such a technique in Yucatan mini pigs using standard vitrectomy instruments, a custom injector for delivery of an iPSC-RPE patch and a clamp for temporary closure of the sclerotomy.

Methods : A four-port pars-plana vitrectomy with posterior vitreous detachment is followed by a posterior retinal detachment and retinotomy. One working port is enlarged to fit the injector and the iPSC-RPE-patch released in the subretinal space using foot pedal control. The sclerotomy is temporally closed using the clamp and a fluid air exchange performed to provide surface tension management and prevent patch migration through the retinotomy. The sclerotomy is sutured under air.

Results : 27 surgeries were performed in 14 animals. 21 surgeries (77.7%) ended up without complications. Most common complications were vitreous hemorrhage, PVR, and retinal detachment. The lightweight ergonomic design of the injector together with the foot pedal control through the viscous fluid injector allowed a smooth delivery of the patch. Temporary closure of the sclerotomy using the custom clamp prevented depressurization of the eye when the injector is withdrawn.

Conclusions : The surgical technique together with the use of a custom injector and clamp allowed a safe and reproducible delivery of the iPSC-RPE-patch. This technique will be applied in upcoming phase I clinical trial for treating patients with the dry-form of AMD.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×