July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Feasibility of detecting RNA expression in human vitreous
Author Affiliations & Notes
  • Michelle Peng
    University of California, San Francisco, San Francisco, California, United States
  • Kornwipa Hemarat
    University of California, San Francisco, San Francisco, California, United States
  • Qingyun Liu
    University of California, San Francisco, San Francisco, California, United States
  • Xiangbin Kong
    University of California, San Francisco, San Francisco, California, United States
  • Intira Sukpen
    University of California, San Francisco, San Francisco, California, United States
  • Max Kudish
    University of California, San Francisco, San Francisco, California, United States
  • James Lo
    University of California, San Francisco, San Francisco, California, United States
  • Diego Tapas
    University of California, San Francisco, San Francisco, California, United States
  • Catherine Chia
    University of California, San Francisco, San Francisco, California, United States
  • Jacquelyn Kemmer
    University of California, San Francisco, San Francisco, California, United States
  • Ariana Naaseh
    University of California, San Francisco, San Francisco, California, United States
  • Thuy Doan
    University of California, San Francisco, San Francisco, California, United States
  • Ricardo Lamy
    University of California, San Francisco, San Francisco, California, United States
  • Jay M Stewart
    University of California, San Francisco, San Francisco, California, United States
  • Footnotes
    Commercial Relationships   Michelle Peng, None; Kornwipa Hemarat, None; Qingyun Liu, None; Xiangbin Kong, None; Intira Sukpen, None; Max Kudish, None; James Lo, None; Diego Tapas, None; Catherine Chia, None; Jacquelyn Kemmer, None; Ariana Naaseh, None; Thuy Doan, None; Ricardo Lamy, None; Jay Stewart, None
  • Footnotes
    Support  That Man May See, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 334. doi:
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      Michelle Peng, Kornwipa Hemarat, Qingyun Liu, Xiangbin Kong, Intira Sukpen, Max Kudish, James Lo, Diego Tapas, Catherine Chia, Jacquelyn Kemmer, Ariana Naaseh, Thuy Doan, Ricardo Lamy, Jay M Stewart; Feasibility of detecting RNA expression in human vitreous. Invest. Ophthalmol. Vis. Sci. 2018;59(9):334.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Vitreoretinal conditions are often due to a complex interplay of risk factors and molecular events that have not been well elucidated. Understanding these molecular pathways may lead to improved treatment algorithms for patients.

Methods : We aim to qualitatively identify known markers for diabetes: vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) in human vitreous specimen from patients at the University of California San Francisco and San Francisco General Hospital. Ribonucleic acid (RNA) extraction, reverse transcription, and quantitative polymerase chain reaction for VEGF, IL-6, and MCP-1 were performed in triplicate at a later date.

Results : Five vitreous specimens from proliferative diabetic retinopathy (PDR) patients and 10 with vitreous opacities (VO) or epiretinal membrane (ERM) were analyzed. RNA expression of VEGF, IL-6, MCP-1 was higher in eyes with PDR in comparison with VO and ERM.

Conclusions : This study shows that RNA isolation from human vitreous samples is feasible. Qualitatively, we demonstrate increased expression of genes known to play a part in pathogenesis of patients with PDR.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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