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Miao Zhang, Guangyu Li, Meng Shi, Lu Chen; Novel characterization and intravital imaging of dynamic conjunctival lymphangiogenesis after filtration surgery. Invest. Ophthalmol. Vis. Sci. 2018;59(9):480.
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© ARVO (1962-2015); The Authors (2016-present)
Glaucoma is a leading cause of blindness that affects many people worldwide. Filtration surgery is a common procedure to lower IOP (intraocular pressure) and treat glaucoma. In this study, we investigated the effect of filtration surgery on conjunctival lymphatic vessels using the fluorescently labeled transgenic mice of Prox-1, the master control gene for lymphatic development.
Glaucoma filtration surgery was performed on transgenic mice of Prox-1-GFP (green fluorescent protein). Conjunctival follicular areas were examined in vivo for two weeks by an advanced live imaging system, ophthalmic slit-lamp biomicroscopy and OCT (optical coherence tomography). IOP was measured by a non-invasive TonoLab tonometer. Additionally, conjunctival tissues after the filtration surgery were immunostained for LYVE-1 (lymphatic vessel endothelial hyaluronan receptor 1), a lymphatic specific marker.
IOP was lowered in the eyes after the filtration surgery. Newly formed Prox-1 positive vessels were observed in the follicular areas. These vessels elongated over our observational time window of two weeks after the surgery. The Prox-1 positive vessels also expressed LYVE-1, further confirming their lymphatic property.
This study provides the first evidence showing real-time and intravital processes of lymphangiogenesis in the conjunctiva after the filtration surgery. Further investigation may reveal new mechanisms and lymphatic-based therapy for glaucoma.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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