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Akira Matsuda, Yosuke Asada, Satoshi Iwamoto; The alteration of gene expression profiles after filtration surgery in mouse. Invest. Ophthalmol. Vis. Sci. 2018;59(9):493.
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© ARVO (1962-2015); The Authors (2016-present)
Fibrotic changes in bleb region after filtration surgery often induce re-elevation of intraocular pressure. To clarify the alteration of gene expression profiles after filtration surgery, we carried out microarray expression analysis using mouse models of filtration surgery.
Using BALB/c mouse, filtration surgery model was made as previously reported (ARVO 2016). The bleb region tissue was sampled 3 days and 7 days after filtration surgery using 2mm biopsy punches. After total RNA extraction, gene array analysis was carried out using Agilent 8X60K mouse expression array.
At day 3 after surgery the bleb is maintained and at 7days after surgery, the bleb region became encapsulated. We find 789 and 871 overexpressed genes in the bleb region compared the control conjunctiva at 3days/7days after filtration surgery. Gene ontology analysis showed activation of T lymphocyte/neutrophil migration pathways, and collagen synthesis pathways. Hyperexpression of S100a8/S100a9, keratin 16, Il1beta genes, and downregulation of Muc5ac and Gp2 genes were also observed.
Our results showed activation of wound healing-related genes and downregulation of homeostatic genes for conjunctival epithelium after filtration surgery. The results suggested importance of epithelial wound healing after filtration surgery.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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