July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Human HSV-Specific Memory CD8+ TEM Cells with Unique JAK/STAT, Chemokine and Anti-Inflammatory Gene Signatures Are Associated with Asymptomatic Ocular Herpes Infection
Author Affiliations & Notes
  • Lbachir BenMohamed
    Gavin Herbert Eye Institute, Univ of California-Irvine, Irvine, California, United States
  • Hawa Vahed
    Gavin Herbert Eye Institute, Univ of California-Irvine, Irvine, California, United States
  • Ruchi Srivastava
    Gavin Herbert Eye Institute, Univ of California-Irvine, Irvine, California, United States
  • Anthony B Nesburn
    Gavin Herbert Eye Institute, Univ of California-Irvine, Irvine, California, United States
  • Footnotes
    Commercial Relationships   Lbachir BenMohamed, None; Hawa Vahed, None; Ruchi Srivastava, None; Anthony Nesburn, None
  • Footnotes
    Support  This work is supported by Public Health Service Research R01 Grants EY026103, EY019896 and EY024618 from National Eye Institute (NEI) and R21 Grant AI110902 from National Institutes of allergy and Infectious Diseases (NIAID), by The Discovery Center for Eye Research (DCER) and by a Research to Prevent Blindness (RPB) unrestricted departmental grant
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 519. doi:
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      Lbachir BenMohamed, Hawa Vahed, Ruchi Srivastava, Anthony B Nesburn; Human HSV-Specific Memory CD8+ TEM Cells with Unique JAK/STAT, Chemokine and Anti-Inflammatory Gene Signatures Are Associated with Asymptomatic Ocular Herpes Infection. Invest. Ophthalmol. Vis. Sci. 2018;59(9):519.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : A large proportion of the world population harbors herpes simplex virus type 1 (HSV-1) which causes potentially blinding recurrent herpetic disease. HSV-specific CD8+ T cells provide immunosurveillance of latently infected sensory neurons from which the virus sporadically reactivates from latency causing recurrent herpetic disease. However, the molecular signatures of CD8+ T cells that protect asymptomatic (ASYMP) individuals who never experienced any recurrent herpetic disease, remain to be elucidated.

Methods : In this study, we used Flow Cytometry and NanoString assays to compare the phenotype, the effector function, and the expression of a comprehensive panel of 579 immune genes of memory CD8+ T cells, that shared the same epitope-specificity, but were freshly sorted from the peripheral blood of well-characterized cohorts of ASYMP and symptomatic (SYMP) individuals, with a history of numerous episodes of recurrent herpetic disease.

Results : Regardless of the epitope specificity: (i) we detected frequent multi-functional HSV-specific effector memory CD62LlowCD44highCD8+ TEM cells in ASYMP individuals compared to more of central memory CD62LhighCD44highCD8+ TCM cells in SYMP individuals; (ii) we found memory CD8+ T cells from ASYMP individuals express higher level of JAK/STAT, chemokine and anti-inflammatory genes.

Conclusions : These findings suggest that expansion of effector function of HSV-specific memory CD8+ TEM cells with molecular signatures associated with T cell activation, migration and anti-inflammation play an important role in asymptomatic herpes infection in humans.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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