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Lbachir BenMohamed, Hawa Vahed, Ruchi Srivastava, Anthony B Nesburn; Human HSV-Specific Memory CD8+ TEM Cells with Unique JAK/STAT, Chemokine and Anti-Inflammatory Gene Signatures Are Associated with Asymptomatic Ocular Herpes Infection. Invest. Ophthalmol. Vis. Sci. 2018;59(9):519.
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© ARVO (1962-2015); The Authors (2016-present)
A large proportion of the world population harbors herpes simplex virus type 1 (HSV-1) which causes potentially blinding recurrent herpetic disease. HSV-specific CD8+ T cells provide immunosurveillance of latently infected sensory neurons from which the virus sporadically reactivates from latency causing recurrent herpetic disease. However, the molecular signatures of CD8+ T cells that protect asymptomatic (ASYMP) individuals who never experienced any recurrent herpetic disease, remain to be elucidated.
In this study, we used Flow Cytometry and NanoString assays to compare the phenotype, the effector function, and the expression of a comprehensive panel of 579 immune genes of memory CD8+ T cells, that shared the same epitope-specificity, but were freshly sorted from the peripheral blood of well-characterized cohorts of ASYMP and symptomatic (SYMP) individuals, with a history of numerous episodes of recurrent herpetic disease.
Regardless of the epitope specificity: (i) we detected frequent multi-functional HSV-specific effector memory CD62LlowCD44highCD8+ TEM cells in ASYMP individuals compared to more of central memory CD62LhighCD44highCD8+ TCM cells in SYMP individuals; (ii) we found memory CD8+ T cells from ASYMP individuals express higher level of JAK/STAT, chemokine and anti-inflammatory genes.
These findings suggest that expansion of effector function of HSV-specific memory CD8+ TEM cells with molecular signatures associated with T cell activation, migration and anti-inflammation play an important role in asymptomatic herpes infection in humans.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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