July 2018
Volume 59, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2018
Plasminogen promotes phagocytic activity of cultured human corneal fibroblasts
Author Affiliations & Notes
  • Tomoko Sato
    Kindai University Faculty of Medicine , Osaka-sayama, Japan
  • Koji Sugioka
    Kindai University Faculty of Medicine , Osaka-sayama, Japan
  • Junko Murakami
    Kindai University Faculty of Medicine , Osaka-sayama, Japan
  • Hiroshi Mishima
    Kindai University Faculty of Medicine , Osaka-sayama, Japan
  • Teruo Nishida
    Ophthalmology, Yamaguchi University, Ube, Japan
  • Yoshikazu Shimomura
    Kindai University Faculty of Medicine , Osaka-sayama, Japan
  • Footnotes
    Commercial Relationships   Tomoko Sato, None; Koji Sugioka, None; Junko Murakami, None; Hiroshi Mishima, None; Teruo Nishida, None; Yoshikazu Shimomura, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 525. doi:
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    • Get Citation

      Tomoko Sato, Koji Sugioka, Junko Murakami, Hiroshi Mishima, Teruo Nishida, Yoshikazu Shimomura; Plasminogen promotes phagocytic activity of cultured human corneal fibroblasts. Invest. Ophthalmol. Vis. Sci. 2018;59(9):525.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Corneal fibroblasts (CF) have a high phagocytic activity. The phagocytic function of CF plays an important role in corneal stromal wound healing. In this study we examined the effect of plasminogen on phagocytosis by CF.

Methods : The ability of plasminogen to bind CF was measured with a real-time cell-molecular interaction assay system, IAsys resonant mirror biosensor. To investigate the phagocytic activity, plastic beads labeled with FITC were used in this study. CF were incubated in media containing plasminogen and plastic beads for 2 hours or 24 hours. The effects of plasminogen on phagocytic activity of CF was measured by flow cytometry. To clarify the mechanism of phagocytic activity by plasminogen, we used 6-Aminohexanoic Acid (EACA) which blocks plasminogen binding to receptor on cell surface.

Results : The binding response of plasminogen to CF was increased in a time-dependent and cell number dependent fashions. For flow cytometry analysis, plasminogen did not promote phagocytic activity at 2 hours after incubation(2.5±1.9%vs. 4.1±1.5%, p<0.01). However, at 24 hours after incubation, plasminogen significantly promoted phagocytic activity(1.9±1.4%vs. 11.7±3.8%, p<0.01). EACA completely inhibited the phagocytic activity stimulated by plasminogen(4.0±0.6%).The binding response of plasminogen to CF was increased in a time-dependent and cell number dependent fashions. For flow cytometry analysis, plasminogen did not promote phagocytic activity at 2 hours after incubation(2.5±1.9%vs. 4.1±1.5%, p<0.01). However, at 24 hours after incubation, plasminogen significantly promoted phagocytic activity(1.9±1.4%vs. 11.7±3.8%, p<0.01). EACA completely inhibited the phagocytic activity stimulated by plasminogen(4.0±0.6%).

Conclusions : These data demonstrate that plasminogen promotes phagocytic activity of CF. The phagocytic activity by plasminogen depended on the duration of the incubation period. Plasminogen binding to CF is critical to exert its phagocytic activity.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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