July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Intravitreal injection, but not topical instillation, of atropine inhibits myopic shift in the chick lens-induced myopia model
Author Affiliations & Notes
  • Takaaki Inaba
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Tomoko Oda
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Toru Shibata
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Hitomi Kamio
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Kenji Ueda
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Masatomo Kato
    Santen Pharmaceutical Co., Ltd., Nara, Japan
  • Jin-Zhong Zhang
    Santen Pharmaceutical Co., Ltd., Nara, Japan
    Santen Inc., Emeryville, California, United States
  • Footnotes
    Commercial Relationships   Takaaki Inaba, Santen Pharmaceutical Co, Ltd. (E); Tomoko Oda, Santen Pharmaceutical Co., Ltd. (E); Toru Shibata, Santen Pharmaceutical Co, Ltd. (E); Hitomi Kamio, Santen Pharmaceutical Co, Ltd. (E); Kenji Ueda, Santen Pharmaceutical Co, Ltd. (E); Masatomo Kato, Santen Pharmaceutical Co, Ltd. (E); Jin-Zhong Zhang, Santen Pharmaceutical Co, Ltd. (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 690. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Takaaki Inaba, Tomoko Oda, Toru Shibata, Hitomi Kamio, Kenji Ueda, Masatomo Kato, Jin-Zhong Zhang; Intravitreal injection, but not topical instillation, of atropine inhibits myopic shift in the chick lens-induced myopia model. Invest. Ophthalmol. Vis. Sci. 2018;59(9):690.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Myopia is one of the most common eye disorders worldwide and its prevalence is increasing both in children and adolescents. Therefore, controlling myopia progression is an urgent public health goal. We evaluated the effect of atropine by intravitreal (IVT) injection or topical instillation on lens-induced myopia in chicks.

Methods : Right eyes of white leghorn chicks, aged five days, were covered with -9D spectacle lenses for 6 days. Chick received either IVT injections of atropine or saline on days 0, 2 and 4, or topical instillations of atropine once daily during the period. The refractive error and the axial length of the eyes were measured immediately prior to and 6 days after the lens was applied. After sacrifice, eyes underwent pathological analyses. For pharmacokinetics study 10% atropine (eye drops) or 1mM and10 mM atropine (IVT injections) was administered into right eyes, and both eyes were collected per animal at specified time points.

Results : Lens coverage of eyes resulted in myopic changes in refractive error and axial length elongation. IVT injections of 100 mM atropine significantly inhibited both of axial length elongation and refractive error. However, topical instillation of 10% atropine was ineffective with respect to the myopic shift. The scleral concentrations of atropine following topical instillation were lower than those from the IVT injections. Histological analysis indicated differences in anatomical structure between birds and mammals.

Conclusions : Atropine demonstrated anti-myopic effects via IVT injection, but not by topical instillation in this myopia model. The difference in efficacy between IVT injection and topical instillation might be due to anatomical features of the avian model, and the distribution of atropine to target tissues.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×