July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Retinal Simultaneous Competing Defocus of Opposite Sign Induces Up-Regulation of BMP Gene Expression in Chick RPE
Author Affiliations & Notes
  • Yan Zhang
    School of Optometry, University of California, Berkeley, Berkeley, California, United States
  • Sara Yasmin Azmoun
    School of Optometry, University of California, Berkeley, Berkeley, California, United States
  • John Riches Chhoa
    School of Optometry, University of California, Berkeley, Berkeley, California, United States
  • Christine Frances Wildsoet
    School of Optometry, University of California, Berkeley, Berkeley, California, United States
  • Footnotes
    Commercial Relationships   Yan Zhang, None; Sara Azmoun, None; John Chhoa, None; Christine Wildsoet, None
  • Footnotes
    Support  NIH Grant R01EY012392, K12EY017269, K08EY023609
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 752. doi:
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      Yan Zhang, Sara Yasmin Azmoun, John Riches Chhoa, Christine Frances Wildsoet; Retinal Simultaneous Competing Defocus of Opposite Sign Induces Up-Regulation of BMP Gene Expression in Chick RPE. Invest. Ophthalmol. Vis. Sci. 2018;59(9):752.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : This study investigated the effect on bone morphogenetic protein (BMP) gene expression in chick retinal pigment epithelium (RPE) of simultaneous competing retinal defocus of opposite sign.

Methods : 14-day old White-Leghorn chicks wore monocular Fresnel lenses of alternating powers (+10D/-10D) for 15 min, 2 h, and 3 days. qPCR was used to quantify differential gene expression in chick RPE for BMP2, 4, and 7.

Results : Gene expression was up-regulated for BMP2 and BMP4, after short periods of lens wear. BMP2 and BMP4 gene expression was up-regulated 199 ± 11% and 147 ± 26 % respectively after as short as 15 min exposure, with 2 h of treatment inducing much greater changes for both BMP2 (944 ± 244%) and BMP4 (229 ± 31%). However, neither BMP2 or BMP4 showed significant differential gene expression after 3 days of treatment (BMP2, 116 ± 31%; BMP4, 114 ± 21%). The BMP7 gene did not show differential expression under any of the treatment conditions.

Conclusions : Expression of both BMP2 and BMP4 genes in chick RPE is sensitive to retinal defocus manipulations. That both showed early up-regulation with simultaneous competing retinal defocus of opposite sign is consistent with observed slowing of ocular growth, as is also characteristic of imposed myopic defocus alone. Thus RPE-BMP2/4 gene expression changes represent robust predictors of ocular growth changes. The results also further implicate these growth factors in the molecular signaling pathways mediating slowed eye elongation.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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