July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
MIF promoter polymorphism variations in retinal detachment with proliferative vitreoretinopathy
Author Affiliations & Notes
  • Colleen M Cebulla
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Heather Van Law
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
    The Ohio State University College of Optometry, Columbus, Ohio, United States
  • Bongsu Kim
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Rania Kusibati
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Tyler Heisler-Taylor
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Demarcus Williams
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Jay Matthias
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Frederick H. Davidorf
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Matthew Ohr
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Michael Wells
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • Dino Klisovic
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
    Midwest Retina, Dublin, Ohio, United States
  • John B Allen
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
  • William Terrell
    Mary Lanning Healthcare, Hastings, Nebraska, United States
  • Daniel M Miller
    Cincinnati Eye Institute, Cincinnati, Ohio, United States
  • Robert Pilarski
    Division Human Genetics, Ohio State University, Columbus, Ohio, United States
  • Mohamed H Abdel-Rahman
    Ophthalmology and Visual Science, The Ohio State University , Columbus, Ohio, United States
    Division Human Genetics, Ohio State University, Columbus, Ohio, United States
  • Footnotes
    Commercial Relationships   Colleen Cebulla, None; Heather Van Law, None; Bongsu Kim, None; Rania Kusibati, None; Tyler Heisler-Taylor, None; Demarcus Williams, None; Jay Matthias, None; Frederick H. Davidorf, None; Matthew Ohr, None; Michael Wells, None; Dino Klisovic, None; John B Allen, None; William Terrell, None; Daniel Miller, None; Robert Pilarski, None; Mohamed Abdel-Rahman, None
  • Footnotes
    Support  NIH K08EY022672, P30 CA016058 ( OSU-CCC Nucleic Acids Shared Resource), NCATS KL2TR001068, Ohio Lions Eye Research Foundation
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1156. doi:
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    • Get Citation

      Colleen M Cebulla, Heather Van Law, Bongsu Kim, Rania Kusibati, Tyler Heisler-Taylor, Demarcus Williams, Jay Matthias, Frederick H. Davidorf, Matthew Ohr, Michael Wells, Dino Klisovic, John B Allen, William Terrell, Daniel M Miller, Robert Pilarski, Mohamed H Abdel-Rahman; MIF promoter polymorphism variations in retinal detachment with proliferative vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1156.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Pro-inflammatory factors promote development of proliferative vitreoretinopathy (PVR) in retinal detachment (RD). We tested the hypothesis that promoter polymorphisms that influence expression of the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) are associated with PVR development.

Methods : A multi-center prospective study of 284 American RD patients with PVR (n=114) and without PVR (n= 170) and local unaffected controls (n=202) was conducted under an IRB-approved protocol. Blood samples, vitreous surgical specimens when available, and clinical data were collected. Germline DNA was extracted from mononuclear cells at the OSU Human Cancer Genetic sample bank. Two polymorphisms, rs755622 (previously reported as -173G>C) and rs5844572 (previously reported as -794 (CATT)5-8) were evaluated with allele-specific primer PCR and sequencing; frequencies of genotypes, alleles, and the C7 haplotype were compared in patients with data (n=137 RD and 74 RD-PVR). Chi-square and Fisher exact analysis was performed and odds ratios calculated.

Results : There was no significant difference between the frequency of the MIF polymorphisms in unaffected regional controls and the RD population. In the RD patients, the C allele of rs755622 was found to be less frequent in RD-PVR than in RD (odds ratio [95% confidence intervals]=0.5456 [0.3146-0.9462]; p=0.0310). The rs5844572 7 allele was also lower in RD-PVR patients (5.4%) vs RD (15%) (p=0.0237). Fisher exact test showed a significant decreased frequency of the C7 haplotype in RD-PVR patients compared to RD. The odds ratio of C7 = 0.3401 [0.1488-0.7772]; p=0.0105.

Conclusions : Specific MIF promoter polymorphisms may influence the development of PVR in patients with RD. Further studies to evaluate MIF’s role in the retina are needed.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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