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Hui Chen, Qian Zhang; The protective effect and immune suppressive mechanism of Fingomolid (FTY720) in light-induced retinal damage rats. Invest. Ophthalmol. Vis. Sci. 2018;59(9):975.
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Fingomolid (FTY720), an immunosuppressive agent, was found to protect the retina and suppress the circulatory lymphocytes in the light-induced retinal damage (LIRD) modal in our previous study. Recently it was found the local immune reaction probably play a role in the retinal damage. Whether the local immune of retina was modulated by FTY720 was unknown. The protective effect of FTY720 was further confirmed and its local immune reaction in the retina was investigated in this study.
Sprague-Dawley rats raised in cyclic dim light were exposed to 2700 lux white light for 6 hours. The effect of three single doses (2.5, 5.0, and10.0 mg/kg) by intraperitoneal injection at 0.5 h before the start of light exposure was measured by histological and functional analyses. The apoptosis of retinal cells was detected by TUNEL. The changes of CD3+, CD4+ and CD8+ cells in the retina were measured by immunohistochemistry, and the RNA/protein expression of retina cytokines were measured.
FTY720 treatment preserves rod a-wave responses in a dose-dependent manner, with a dose of 10 mg/kg providing maximal protection. Furthermore, the a-wave, b-wave and OPs-waves in rats treated with FTY720 (10mg/kg) are all significant higher than that in the LIRD group(p<0.001), and 10 mg/kg of FTY720 protects the photoreceptor cells significantly in the histology of retina section and TUNEL assay. CD3+, CD4+ and CD8+ cells in the LIRD retina are suppressed in the immunehistologic assay, while only the protein expression of CD3+ and CD8+ are significantly inhibited.
FTY720 can effectively protect the retina and its function from light-induced damage in rats modal. It can both suppress CD3+, CD4+ and CD8+ cells in retina and inhibit the apoptosis of photoreceptor.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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