July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Comparative transcriptomic and epigenomic analysis identifes regulators of neurogenic competence in retinal Müller glia.
Author Affiliations & Notes
  • Seth Blackshaw
    Neuroscience, Johns Hopkins Univ Sch of Med, Baltimore, Maryland, United States
  • Thanh Hoang
    Neuroscience, Johns Hopkins Univ Sch of Med, Baltimore, Maryland, United States
  • Jie Wang
    Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Manuela Lahne
    Biology, Notre Dame University, South Bend, Indiana, United States
  • Levi Todd
    Neuroscience, Ohio State University Medical Center, Columbus, Ohio, United States
  • Clayton Santiago
    Ophthalmology, University of Florida, Gainesville, Florida, United States
  • Nathalie Squires
    Neuroscience, Ohio State University Medical Center, Columbus, Ohio, United States
  • Patrick Boyd
    Biology, Notre Dame University, South Bend, Indiana, United States
  • John D Ash
    Ophthalmology, University of Florida, Gainesville, Florida, United States
  • Andrew J Fischer
    Neuroscience, Ohio State University Medical Center, Columbus, Ohio, United States
  • Jiang Qian
    Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • David R Hyde
    Biology, Notre Dame University, South Bend, Indiana, United States
  • Footnotes
    Commercial Relationships   Seth Blackshaw, None; Thanh Hoang, None; Jie Wang, None; Manuela Lahne, None; Levi Todd, None; Clayton Santiago, None; Nathalie Squires, None; Patrick Boyd, None; John Ash, None; Andrew Fischer, None; Jiang Qian, None; David Hyde, None
  • Footnotes
    Support  U01EY027267
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 991. doi:https://doi.org/
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    • Get Citation

      Seth Blackshaw, Thanh Hoang, Jie Wang, Manuela Lahne, Levi Todd, Clayton Santiago, Nathalie Squires, Patrick Boyd, John D Ash, Andrew J Fischer, Jiang Qian, David R Hyde; Comparative transcriptomic and epigenomic analysis identifes regulators of neurogenic competence in retinal Müller glia.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):991. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : A potential approach for restoring vision in patients with photoreceptor dystrophies is the regeneration of lost retinal neurons from Müller glia. To identify molecular mechanisms that control neurogenic competence in Müller glia, we have carried out a large-scale comparative transcriptomic and epigenomic analysis using zebrafish (regeneration-competent), chick (regeneration-limited), and mouse (regeneration-refractory).

Methods : We have used RNA-Seq, miRNA-Seq and ATAC-Seq to comprehensively profile changes in mRNA and miRNA expression, as well as chromatin conformation, that occur during differentiation of Müller glia from retinal progenitor cells, and following both inner and outer retinal damage, as well as growth factor treatment, in all three species.

Results : These data have identified many candidate genes and transcriptional regulatory networks that either promote or restrict neurogenic competence in Müller glia.

Conclusions : These findings may help identify molecular pathways that could be targeted to induce neurogenic competence in human Müller glia.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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