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Leticia Vazquez-Maya, David Gomez-Garcia, Jose Francisco Francisco Perez-Vazquez, Karla Paola Garcia-Carmona, Leticia Carrete-Gonzalez; CONJUNCTIVAL FUNDUS CULTURES IN DONOR CORNEAS (BACTERIOLOGICAL LOAD DETECTION). Invest. Ophthalmol. Vis. Sci. 2018;59(9):1349.
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The aim of this study was to determine through culture, the types of microorganisms present in conjunctival fundus of cadaveric donor corneal patients, after the use of antiseptics and moxifloxacin, as well as to determine if there is development of them in the preservation media used for donor tissue.
Descriptive, prospective, analytical, observational study, from March 2001 to October 2014 at Hospital General de México “Dr. Eduardo Liceaga”. Conjunctival fundus cultures from 70 cornea donors were taken. With sterile technique, the first culture after the placement of iodopovidone, the second culture subsequent to moxifloxacin and a third culture of the corneal preservation medium, they were inoculated in Agar plates, reading the results and identifying with WalkAway 96 equipment (MicroScan system). The culture of the microorganism was considered positive when there are more than 20 CFU. An analysis of square chi was made.
In phase 1 it was found that 28 cultures (20%) developed some germ in a significant way. As a result of phase 2 it was found that 10 cultures (7%) developed some germ. No microorganism was developed in phase 3 of the study.The most common microorganism found in phase 1 was Staphylococcus epidermidis in 11 cultures (38%), Pseudomonas aeruginosa in 11%, Candida / Klebsiella in 7%, Citrobacter amalonaticus in 7% and Streptococcus viridans in 4%; Acinetobacter baumanni / haemolyticus in 7%, Empedobacter (f) brevis in 4%; S. aureus in 7%.The most common microorganism found in phase 2 with 30% was Staphylococcus epidermidis, with Pseudomonas aeruginosa in 30%, another 20% with Candida/ Klebsiella and 20% with Citrobacter amalonaticus.In phase 3 all the cultures were negative.The statistical analysis reported a value of p = 0.03 (p <0.05) considered statistically significant.
Asepsis and antisepsis contributes to the eradication of a large number of germs of the normal and abnormal flora of the conjunctiva, and together with the instillation of a broad-spectrum antibiotic, greatly enhances the optimal state of the corneal tissue. The corneal preservation medium enriched with antibiotics (Optisol GS) eradicates 100% of the microorganisms regardless of the time of preservation.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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