July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Effect of SOX2 Overexpression on Regeneration of Corneal Endothelial Cells
Author Affiliations & Notes
  • Young Joo Shin
    Ophthalmology, Hallym University College of Medicine, Seoul, Korea (the Democratic People's Republic of)
  • Hyun Seung Kim
    Department of Ophthalmology and Visual Science, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Jin Sun Hwang
    Ophthalmology, Hallym University College of Medicine, Seoul, Korea (the Democratic People's Republic of)
  • Tae-Young Chung
    Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Yoon Kyung Chang
    Ophthalmology, Hallym University College of Medicine, Seoul, Korea (the Democratic People's Republic of)
  • Footnotes
    Commercial Relationships   Young Joo Shin, None; Hyun Seung Kim, None; Jin Sun Hwang, None; Tae-Young Chung, None; Yoon Kyung Chang, None
  • Footnotes
    Support  This study was supported by the National Research Foundation (NRF) grant (NRF-2015R1D1A1A09058505) funded by the Korea government.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1370. doi:
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    • Get Citation

      Young Joo Shin, Hyun Seung Kim, Jin Sun Hwang, Tae-Young Chung, Yoon Kyung Chang; Effect of SOX2 Overexpression on Regeneration of Corneal Endothelial Cells
      . Invest. Ophthalmol. Vis. Sci. 2018;59(9):1370.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : In this study, we investigated whether overexpression of sex-determining region Y-box 2 (SOX2) regenerates human corneal endothelial cells (HCECs) in vitro.

Methods : SOX2 was overexpressed by CRISPR/dCAS9 activation systems. HCECs were cultured as previously described. Cell viability, proliferation rate, cell cycle analysis was analyzed according to SOX2 overexpression. Western blotting and RT-PCR were performed to evaluate the protein and mRNA expression.

Results : Cell viability, proliferation rate and the number of cells in S phase were increased by SOX2 overexpression (p<0.05). Cyclin-dependent kinase1 and cyclin D1 were overexpressed and collagen VIIIa2 expression was decreased by SOX2 overexpression (p<0.05). Wnt signaling was repressed and AKT pathway was activated by SOX2 overexpression. Mitochondrial oxidative stress and adenosine triphosphate production was increased by SOX2 overexpression (p<0.05).

Conclusions : SOX2 overexpression may contribute to wound healing and regeneration of HCECs. SOX2 overexpression may be useful in treatment of HCECs disease

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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