July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Experimental study of skin-derived corneal endothelial cell-like cells for long-term monkey corneal endothelial transplantation

Author Affiliations & Notes
  • Xinyi Wu
    Ophthal QiLu Hosp/Ophthal, Shandong University, Jinan, SHANDONG, China
  • Footnotes
    Commercial Relationships   Xinyi Wu, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1381. doi:
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      Xinyi Wu; Experimental study of skin-derived corneal endothelial cell-like cells for long-term monkey corneal endothelial transplantation

      . Invest. Ophthalmol. Vis. Sci. 2018;59(9):1381.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Explore the feasibility of skin-derived corneal endothelial cell-like cells (CEC-like cells) to cure monkey corneal endothelial dysfunction.

Methods : Human skin stem cells were differentiated into CEC-like cells in our previous protocol. CEC-like cells were transplanted into the monkey corneal endothelial dysfunction models to detect the cell function in vivo. Slip-lamp, optical coherence tomography, non-contact specular microscopy, B-mode ultrasound and fundus photography were carried out in long-term observation. Histopathological and immunofluorescent examinations were carried to detect the CEC-like cells.

Results : CEC-like cells had similar morphology and characteristic to CECs. CEC-like cells were transplanted into the monkey corneal endothelial dysfunction models, corneas became significantly clearly transparent that the pupil and iris texture could be seen, while the corneas of the control group were cloudy and thick. One year later, the cornea of the CEC-like cell group were still transparent while the corneal opacity and stromal edema were still serious in the control group. There were no new precipitates or vessels appeared during observation. Polygonal CEC-like cells closely connected to each other in the form of a monolayer mosaic structure with a cell density about 3000 cell/mm2. B-mode ultrasound and fundus photography showed no pathological changes of the surgical eyes. Na+/K+ ATPase were also expressed in CEC-like cells, indicating the persistent pump function. HE staining showed that the cornea was almost normal and the CEC-like cells tightly connected to each other on the Descemet’s membrane and there were little inflammatory cells in the cornea. In addition, no obvious tissue abnormalities were found in other parts of postoperative monkey.

Conclusions : Skin-derived CEC-like cells have long-term therapeutic effect in monkey corneal endothelial dysfunction model. The renewable cell source and novel deriving method may lead to potential applications in cell replacement therapy for corneal endothelial dysfunction.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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