July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Cytoprotective effect of ALG-1001 peptide (Luminate) on human retinal pigment epithelial cells exposed to oxidative injury. A novel functional-outcome for an anti-VEGF agent.
Author Affiliations & Notes
  • Marco A Beltran
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Ruben Zamora-Alvarado
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Roberto Gonzalez-Salinas
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Rosario Gulias-Cañizo
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Hugo Quiroz-Mercado
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • LUIS F HERNANDEZ
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Hampar Karageozian
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Vicken H Karageozian
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • John Y Park
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Lenin Ochoa-de la Paz
    research department, Asociación Para Evitar la Ceguera, Mexico, Mexico
  • Footnotes
    Commercial Relationships   Marco Beltran, None; Ruben Zamora-Alvarado, None; Roberto Gonzalez-Salinas, None; Rosario Gulias-Cañizo, None; Hugo Quiroz-Mercado, Allegro Ophthalmics (I); LUIS F HERNANDEZ, None; Hampar Karageozian, Allegro Ophthalmics (I); Vicken Karageozian, Allegro Ophthalmics (I); John Park, Allegro Ophthalmics (I); Lenin Ochoa-de la Paz, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1465. doi:
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      Marco A Beltran, Ruben Zamora-Alvarado, Roberto Gonzalez-Salinas, Rosario Gulias-Cañizo, Hugo Quiroz-Mercado, LUIS F HERNANDEZ, Hampar Karageozian, Vicken H Karageozian, John Y Park, Lenin Ochoa-de la Paz; Cytoprotective effect of ALG-1001 peptide (Luminate) on human retinal pigment epithelial cells exposed to oxidative injury. A novel functional-outcome for an anti-VEGF agent.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1465.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The main purpose of this study was to determine the cytoprotective effect of the ALG-1001 peptide on cultured retinal pigment epithelial cells exposed to oxidative stress.

Methods : Human retinal pigment epithelium cells (ARPE-19) were exposed to cellular damage conditions employing hydrogen peroxide (H2O2). For control conditions, cells were exposed to culture medium only, with or without ALG-1001 (1mg) or H2O2 (100 µM) for 24 h. For the ALG-1001 treatment, we followed two strategies: 1) cells culture were exposed to ALG-1001 (1mg) for 24 h, then the medium was remove and new medium with H2O2 (100 µM) without peptide was applied; 2) cells culture were exposed to H2O2 (100 µM) for 24 h, then the medium was remove and applied new medium with ALG-1001 (1mg) without H2O2. Number and the viability of cultured cells was measured using WST-8 and LDH assays. Students-t test was used for statistical analysis.

Results : Exposition to H2O2, decreased the number and viability of cultured cells in a 40 ± 1.0 % and 49 ± 1.3 %, respectively (P<0.05). However, LDH assay does not show a significant effect (8 ± 1.1%) under cellular damage conditions. When the ALG-1001 peptide was added 24 h before the H2O2 exposition, we did not observe a significant reduction in the number (5 ± 1.2 %) and viability (10 ± 1.5 %) of the cell exposed to H2O2. However, when the ALG-1001 was added 24 h after the H2O2 exposition, the cell number and viability, was reduce only 20 ± 1.1 % and 14 ± 1.0 % respectively.

Conclusions : Our findings showed a clear reduction in the toxic effect generated by oxidative stress exposure in cultured cells. Moreover, the compound cytoprotective effect is observed by maintaining cells survival, which is reflected in the number of cells and their metabolic activity. This effect is more evident when the ALG-1001 is applied previously to oxidative stress. Therefore, considering the viability markers used in this study, it is likely that the ALG-1001 acts modulating an anti-apoptotic pathway. The characterization of this cytoprotective function by ALG-1001 peptide allows, at the therapeutic level, not only to decrease the angiogenesis process, but also to maintain cell viability under pathological conditions, a feature that, to our knowledge, has not been observed on any other molecule with anti-integrin activity.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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