July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Electrostimulation promotes Muller glial cell proliferation
Author Affiliations & Notes
  • Sam Enayati
    Department of Ophthalmology, Harvard medical school, Schepens Eye Research Institute, Boston, Massachusetts, United States
    Department of medical biochemistry , Oslo University hospital, Oslo, Norway
  • Kin-Sang Cho
    Department of Ophthalmology, Harvard medical school, Schepens Eye Research Institute, Boston, Massachusetts, United States
  • Tor Paaske Utheim
    Department of medical biochemistry , Oslo University hospital, Oslo, Norway
    Department of Ophthalmology, Harvard medical school, Schepens Eye Research Institute, Boston, Massachusetts, United States
  • Dong Feng Chen
    Department of Ophthalmology, Harvard medical school, Schepens Eye Research Institute, Boston, Massachusetts, United States
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1482. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Sam Enayati, Kin-Sang Cho, Tor Paaske Utheim, Dong Feng Chen; Electrostimulation promotes Muller glial cell proliferation
      . Invest. Ophthalmol. Vis. Sci. 2018;59(9):1482.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Evidence is emerging that non-invasive electrostimulation (ES) improves visual function in certain untreatable blinding conditions, such as retinitis pigmentosa and optic nerve trauma. Our previous study suggests that this effect of ES may associate with its ability to promote the proliferative potential of Muller glia (MG). We here examined the optimized ES condition that drives the intracellular events and gene expression of Muller glia towards a progenitor like state to promote their regenerative potential

Methods : Retinal cells isolated from neonatal B6 mice aged postnatal day 6-7 (P6-7) were dissociated with papain and incubated for 14 days in DMEM/F12. Muller glia were then seeded on a poly-D-lysine coated cover glass. ES of 4 different parameters (frequency, current, duration and waveform), each with 4 different conditions were applied. Proliferation of MG was quantified by 5-ethynyl-2’-deoxyuridine (EdU) incorporation and immunohistochemistry. MG expression and induction of progenitor cell genes were assessed by quantitative reverse transcriptase-polymerase chain reaction (qPCR).

Results : We found that among the different waveforms, negative ramp of ES was most effective and induced a nearly 2-fold increase in MG proliferation as compared to controls. Moreover, variations of ES frequency and current also showed significant impacts on MG proliferation, gene expression, and their progenitor cell potential

Conclusions : ES, particularly the ramp stimulation, enhances the proliferative and progenitor cell potential of MG. Our results suggest the possibility of manipulating MG behavior and neuroregeneration by ES. Further studies are needed to uncover the underlying mechanisms of ES-induced cellular changes.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×